ArticleViruses2025
Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene.
Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Establishment of a Quadruplex RT-qPCR Method for the Detection of All Lineages of PPRV.Animals : an open access journal from MDPI · 2026Article
- Seroprevalence and Associated Risk Factors of Peste des Petits Ruminants Virus in Small Ruminants of Punjab, Pakistan.Transboundary and emerging diseases · 2026Article
- Diagnostic and vaccination challenges in the PPR pre-eradication era: Identifying gaps and potential unintended consequences.Journal of advanced veterinary and animal research · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Peste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prevalence, suggesting a competitive advantage in viral transmission and adaptability. Moreover, a lineage IV strain was the only confirmed strain in Europe and Asia. In this study, a one-step Taqman quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay for lineage IV PPRV was established by targeting the hemagglutinin (H) gene. The results indicated that this method could detect approximately six copies of PPRV RNA, indicating high sensitivity. No cross-reactions with related viruses or other lineages of PPRV were observed. The results of a repeatability test indicated that the coefficient of variation values were low in both the inter-assay and intra-assay experimental groups. Detection of field samples indicated that all positive samples could be detected successfully using the developed method. This RT-qPCR assay provides a valuable tool to facilitate targeted surveillance and rapid differential diagnosis in regions with active circulation of PPRV lineage IV, enabling timely epidemiological investigations and strain-specific identification.
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Registered trials
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