Evidence map›Paper›PMID 40733498›Full record

ArticleViruses2025

The Differential Expression of the JAK/STAT Pathway in Breast Cancer Cells Transfected with Human Papillomavirus Oncogenes.

Stephanie Loureiro Leão, Gabriel Rômulo Parente da Silva, Daffany Luana Dos Santos, Bianca de França São Marcos, Pedro Henrique Bezerra Fontes, Beatriz Eda de Oliveira Isídio, Isabelle Silva Simões, Elisa Fotin Genn Barros, David Beltrán Lussón, Joelson Germano Crispim and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Stephanie Loureiro LeãoLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Gabriel Rômulo Parente da SilvaLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Daffany Luana Dos SantosLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Bianca de França São MarcosLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Pedro Henrique Bezerra FontesLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.ORCID 0000-0001-5609-2977
Beatriz Eda de Oliveira IsídioLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Isabelle Silva SimõesLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Elisa Fotin Genn BarrosLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
David Beltrán LussónLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Joelson Germano CrispimLaboratory for Immunomodulation and New Therapeutic Approaches, Research Center for Therapeutic Innovation, Federal University of Pernambuco, Recife 50670-901, Pernambuco, Brazil.ORCID 0000-0002-3057-1299
Lígia Rosa Sales LealLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Anna Jéssica Duarte SilvaLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.ORCID 0000-0002-8341-7877
Vanessa Emanuelle Pereira SantosLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.
Antonio Carlos de FreitasLaboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Recife 50670-901, Pernambuco, Brazil.ORCID 0000-0002-4957-9549

Funding

National Council for Scientific and Technological Development 308684/2023-0National Council for Scientific and Technological Development 444156/2023-2National Council for Scientific and Technological Development 444606/2023-8
6 · The paper itself

Abstract

Breast cancer is among the most prevalent and deadly types of cancer worldwide. Viral infections have been investigated as contributing factors in breast carcinogenesis, including infections by high-risk genotypes of human papillomavirus (HPV). Although viral DNA has been detected in breast tumors, the role of HPV activity in this type of cancer remains poorly understood. HPV oncogenes interact with various host genes, including those involved in the JAK/STAT signaling pathway. This pathway is associated with the regulation of gene expression related to the tumor microenvironment, and understanding how HPV oncogenes interact with JAK/STAT components may provide insights into the relationship between the virus and breast cancer development. In this study, we assessed the differential expression of the JAK/STAT pathway in MDA-MB-231 cells individually transfected with the E5, E6, and E7 oncogenes of HPV16. The results revealed downregulation of STAT4 in the presence of the E5, E6, and E7 oncogenes. Notably, cells transfected with E5 alone exhibited upregulation of JAK2, STAT3, and STAT6, whereas transfection with E6 and E7 resulted in their downregulation. These findings highlight the underexplored role of the E5 oncogene in contrast to the more extensively studied E6 and E7. Our results support the hypothesis that HPV oncogenes actively modulate the expression of genes involved in the tumor microenvironment in breast cancer.

Indexed as

Breast NeoplasmsHuman papillomavirus 16Janus KinasesOncogene Proteins, ViralPapillomavirus InfectionsSignal TransductionSTAT Transcription FactorsCell Line, TumorFemaleGene Expression Regulation, NeoplasticHuman Papillomavirus VirusesHumansOncogenesPapillomavirus E7 ProteinsRepressor ProteinsTransfectionE6 protein, Human papillomavirus type 16Janus KinasesOncogene Proteins, ViralPapillomavirus E7 ProteinsRepressor ProteinsSTAT Transcription FactorsHPVmammary carcinogenesistumor microenvironment

Identifiers

PMID40733498
PMCPMC12300927

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.