Specific Nested PCR for the Detection of 16SrI and 16SrII Group Phytoplasmas Associated with Yellow Leaf Disease of Areca Palm in Hainan, China.

Huiyuan Ge, Xiuli Meng, Zhaowei Lin, Saad Jan, Weiwei Song, Weiquan Qin, Qinghua Tang, Xiaoqiong Zhu

Abstract read

In one paragraph

Article in Plants (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Huiyuan GeDepartment of Plant Pathology and MARA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.ORCID 0009-0002-9139-6428

Xiuli MengCoconut Research Institute/HPDST Hainan Innovation Center of Academician Team, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.

Zhaowei LinCoconut Research Institute/HPDST Hainan Innovation Center of Academician Team, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.

Saad JanCoconut Research Institute/HPDST Hainan Innovation Center of Academician Team, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.

Weiwei SongCoconut Research Institute/HPDST Hainan Innovation Center of Academician Team, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.

Weiquan QinCoconut Research Institute/HPDST Hainan Innovation Center of Academician Team, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.

Qinghua TangCoconut Research Institute/HPDST Hainan Innovation Center of Academician Team, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.ORCID 0000-0001-7828-6174

Xiaoqiong ZhuDepartment of Plant Pathology and MARA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.ORCID 0000-0002-2721-7707

Funding

Hainan Provincial Department of Science and Technology ZDYF2025XDNY118 and ZDYF2022XDNY208Yazhouwan Scientific and Technological Administration of Sanya SYND-2022-36

6 · The paper itself

Abstract

Yellow leaf disease (YLD), caused by the areca palm yellow leaf phytoplasma (APYL), poses a significant threat to the sustainability of the areca palm industry. Timely and accurate detection is essential for effectively diagnosing and managing this disease. This study developed a novel nested PCR system using primers specifically designed from conserved regions of the phytoplasma 16S rDNA sequence to overcome limitations such as false positives often associated with universal nested PCR primers. The resulting primer pairs HNP-1F/HNP-1R (outer) and HNP-2F/HNP-2R (inner) consistently amplified a distinct 429 bp fragment from APYL strains belonging to the 16SrI and 16SrII groups. The detection sensitivity reached 7.5 × 10

Indexed as

Areca catechu L.areca palm yellow leaf phytoplasmamolecular detection

Identifiers

PMID40733381

DOI10.3390/plants14142144

PMCPMC12299459

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.