Evidence map›Paper›PMID 40732137›Full record

ArticleMicroorganisms2025

Development of a Multiplex Real-Time PCR Assay for the Detection of Eight Pathogens Associated with Bovine Respiratory Disease Complex from Clinical Samples.

Fuxing Hao, Chunhao Tao, Ruilong Xiao, Ying Huang, Weifeng Yuan, Zhen Wang, Hong Jia

Abstract read
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
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  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fuxing HaoJiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Chunhao TaoInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Ruilong XiaoInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Ying HuangInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.ORCID 0009-0002-2828-9021
Weifeng YuanInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.ORCID 0000-0002-2782-6510
Zhen WangInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Hong JiaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Funding

Key Technology Innovation Grant Project of Jiangsu Agri-animal Husbandry Vocational College NSF2022ZR03the Agricultural Science and Technology Innovation Program ASTIP-IAS-11,ASTIP-IAS-15the Earmarked Fund for CARS CARS36
6 · The paper itself

Abstract

Bovine respiratory disease complex (BRDC) is one of the primary causes of morbidity, mortality, and economic loss in cattle worldwide. Accurate and rapid identification of causative pathogenic agents is essential for effective disease management and control. In this study, a novel multiplex fluorescence-based quantitative polymerase chain reaction (qPCR) assay was developed for the simultaneous detection of eight major pathogens associated with BRDC. The targeted pathogens included the following: bovine viral diarrhea virus (BVDV), bovine parainfluenza virus type 3 (BPIV3), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BcoV),

Indexed as

bovine respiratory disease complex (BRDC)diagnosismixed infectionmultiplex qPCR

Identifiers

PMID40732137
PMCPMC12300140

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.