Evidence map›Paper›PMID 40732069›Full record

ArticleMicroorganisms2025

Dietary Protein-Induced Changes in Archaeal Compositional Dynamics, Methanogenic Pathways, and Antimicrobial Resistance Profiles in Lactating Sheep.

Maida Mushtaq, Xiaojun Ni, Muhammad Khan, Xiaoqi Zhao, Hongyuan Yang, Baiji Danzeng, Sikandar Ali, Muhammad Hammad Zafar, Guobo Quan

Abstract read
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Maida MushtaqYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650000, China.
Xiaojun NiYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650000, China.
Muhammad KhanYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650000, China.ORCID 0000-0003-1446-1425
Xiaoqi ZhaoYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650000, China.
Hongyuan YangYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650000, China.
Baiji DanzengYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650000, China.
Sikandar AliYunnan International Joint Laboratory of Conservation and Innovative Utilization of Sheep Germplasm Resources, Jindian, Panlong District, Kunming 650000, China.ORCID 0000-0002-4329-3304
Muhammad Hammad ZafarCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225012, China.ORCID 0000-0002-6135-9987
Guobo QuanYunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming 650000, China.ORCID 0000-0002-8167-3623

Funding

The earmarked fund for the China Agriculture Research System CARS-39-08Yunnan International Joint Laboratory of Conservation and Innovative Utilization of Sheep Germplasm Re sources 202403AP140017
6 · The paper itself

Abstract

Dietary protein levels greatly influence gut microbial ecosystems; however, their effects on gut archaea and associated functions in ruminants require further elucidation. This study evaluated the impact of varying dietary protein levels on gut archaeal composition, antimicrobial resistance (AMR) genes, virulence factors, and functional capacities in sheep. Eighteen ewes (Yunnan semi-fine wool breed, uniparous, 2 years old, and averaging 50 ± 2 kg body weight) were randomly assigned to diets containing an 8.5 (low; H_1), 10.3 (medium; H_m), or 13.9% (high; H_h) crude protein level from the 35th day of pregnancy to the 90th day postpartum. The total duration of the experiment was approximately 202 days. A total of nine fecal samples (three from each group) were analyzed via 16S rRNA and metagenomics sequencing. Higher archaeal alpha diversity and richness were observed in the H_m and H_h groups compared to the H_l group (

Indexed as

antimicrobial resistancedietary proteingut archaeametagenomicsmethane emissionsheep

Identifiers

PMID40732069
PMCPMC12298320

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.