Mutations in Genes with a Role in Cell Envelope Biosynthesis Render Gram-Negative Bacteria Highly Susceptible to the Anti-Infective Small Molecule D66.

Samual C Allgood, Calvin A Ewing, Weiping Chu, Steffen Porwollik, Michael McClelland, Corrella S Detweiler

Abstract read

In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Identification and protective efficacy characterization of novel immunogenic antigens for Salmonella Enteritidis vaccines.Poultry science · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Samual C AllgoodDepartment of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.

Calvin A EwingDepartment of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.ORCID 0000-0003-4090-2896

Weiping ChuDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697, USA.

Steffen PorwollikDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697, USA.

Michael McClellandDepartment of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697, USA.

Corrella S DetweilerDepartment of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.ORCID 0000-0002-7783-0876

Funding

Infection-Dependent Vulnerabilities of Gram-negative Bacterial PathogensR01AI168916 · NIAID · UNIVERSITY OF COLORADO · PI Corrella S Detweiler · 2023 to 2026

$2.2M

NIAID NIH HHS R01 AI168916NIH HHS 1K99AI175656-23NIH HHS 1R01AI075093-09NIH HHS 1R01AI168916-23NIH HHS 1R03AI139557-19NIH HHS 1R21AI151979-21NIH HHS 1R21AI171945-23NIH HHS 2K99AI175656-24NIH HHS 2R01AI075093-10NIH HHS 2R01AI168916-24NIH HHS 2R03AI139557-20NIH HHS 2R21AI151979-22NIH HHS 2R21AI171945-24NIH HHS 3K99AI175656-25NIH HHS 3R01AI168916-25

6 · The paper itself

Abstract

Anti-infectives include molecules that target microbes in the context of infection but lack antimicrobial activity under conventional growth conditions. We previously described D66, a small molecule that kills the Gram-negative pathogen

Indexed as

AcrAB-TolCanti-infectiveefflux pumpgmhB/yaeDinner membranelipopolysaccharide (LPS)outer membraneResistance Nodulation cell Division (RND)salmonellaygeG

Identifiers

PMID40732029

DOI10.3390/microorganisms13071521

PMCPMC12299019

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.