Evidence map›Paper›PMID 40731359›Full record

ArticleMicrobiome2025

Soil microbial legacy mediated by buckwheat flavonoids enhances cabbage resistance to clubroot disease.

Jiabing Wu, Shilin Hu, Jing Chen, Lili Zhou, Shengdie Yang, Na Zhou, Lei Wu, Guoqing Niu, Yong Zhang, Xuesong Ren and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Pea-cucumber crop rotation suppressesFrontiers in microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jiabing Wu *College of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China.
Shilin Hu *College of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China.
Jing ChenCollege of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China.
Lili ZhouCollege of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China.
Shengdie YangJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, Nanjing, China.
Na ZhouChongqing Academy of Agricultural Sciences, Chongqing, China.
Lei WuCollege of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China.
Guoqing NiuCollege of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Yong ZhangCollege of Resources and Environment, Interdisciplinary Research Center for Agriculture Green Development in Yangtze River Basin, Southwest University, Chongqing, China.
Xuesong RenCollege of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China.
Qinfei LiCollege of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China.
Jun YuanJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, Nanjing, China. junyuan@njau.edu.cn.
Hongyuan SongCollege of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China. yuahs@163.com.
Jun SiCollege of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, Southwest University, Chongqing, China. sijun@swu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe legacy of plant growth significantly impacts the health of subsequent plants, yet the mechanisms by which soil legacies in crop rotation systems influence disease resistance through rhizosphere plant-microbiome interactions remain unclear. Using a buckwheat-cabbage rotation model, we investigated how microbial soil legacies shape cabbage growth and clubroot disease resistance.

resultsThree-year field trials revealed that buckwheat rotation sustainably reduced clubroot severity by 67%-97%, regardless of pathogen load. Soil sterilization eliminated this suppression, implicating a microbial basis. Using 16S rRNA sequencing, we identified buckwheat-enriched bacterial taxa (Microbacterium, Stenotrophomonas, Ralstonia) that colonized subsequent cabbage roots. Metabolomic profiling pinpointed buckwheat root-secreted flavonoids - 6,7,4'-trihydroxyisoflavone and 7,3',4'-trihydroxyflavone - as key drivers of microbial community restructuring. These flavonoids synergistically enhanced the efficacy of a synthetic microbial community (SynCom1, containing Microbacterium keratanolyticum, Stenotrophomonas maltophilia, and Ralstonia pickettii), boosting disease suppression by 34% in greenhouse trials. Co-application of flavonoids and SynCom1 improved bacterial colonization in root niches. Although SynCom1 partially activated jasmonic acid (JA)-associated defenses, its effectiveness depended primarily on flavonoid-driven microbial recruitment rather than direct immune induction.

conclusionsBuckwheat rotation induces flavonoid-mediated soil microbiomes that prime JA-dependent immunity in subsequent cabbage crops, thereby decoupling disease severity from pathogen load. This study elucidates how specialized metabolites orchestrate cross-crop microbial legacies for sustainable disease control, providing a blueprint for designing rotation systems through precision microbiome engineering. Video Abstract.

Indexed as

BrassicaDisease ResistanceFagopyrumFlavonoidsPlant DiseasesSoil MicrobiologyBacteriaMicrobiotaPlant RootsRhizosphereRNA, Ribosomal, 16SSoilFlavonoidsRNA, Ribosomal, 16SSoilBuckwheatCabbageClubroot diseaseFlavonoidsRotation systemsSoil microbial legacy

Identifiers

PMID40731359
PMCPMC12309073

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.