ArticleBMC plant biology2025
Recombinant tritin protein exhibits antiviral activity against zucchini yellow mosaic virus.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- The Tomato Transcription Factor RAV Affects the Systemic Infection of TYLCV by Interacting With V2.Molecular plant pathology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRibosome-inactivating proteins (RIPs) are a group of proteins known to inhibit protein synthesis and contribute to plant defense responses. Although the antiviral properties of various RIPs have been demonstrated, the antiviral potential of tritin, a type 1 RIP from bread wheat (Triticum aestivum L.), remains unexplored. This study aimed to investigate the antiviral activity of recombinant tritin against zucchini yellow mosaic virus (ZYMV) in zucchini (Cucurbita pepo L.) plants.
methodsThe tritin gene was isolated from the wheat cultivar Kutluk-94, cloned into the pETDuet-1 expression vector, and expressed in Escherichia coli BL21 (DE3) cells. Following induction, recombinant tritin was purified using Ni-NTA affinity chromatography. Antiviral activity was assessed by measuring morphological parameters, disease severity, and the expression levels of the ZYMV Coat Protein (CP) gene and the Pathogenesis-Related 1 (PR1) through quantitative real-time PCR.
resultsTritin-treated plants exhibited significantly lower ZYMV-CP gene expression compared to virus-inoculated controls at 3- and 15- days post-inoculation. Furthermore, PR1 gene expression was upregulated in response to tritin application, suggesting the activation of systemic defense pathways. Morphological assessments revealed dose-dependent phytotoxic effects, including reductions in chlorophyll content and plant growth at higher tritin concentrations.
conclusionThis study represents the first report in demonstrating that recombinant tritin exhibits antiviral activity against ZYMV, reducing viral replication and enhancing defense gene expression in zucchini plants. However, the phytotoxic effects observed at higher concentrations suggest the need for dose optimization before agricultural application. These findings provide a promising basis for the development of RIP-based antiviral strategies to improve crop tolerance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.