Evidence map›Paper›PMID 40731076›Full record

ArticleArchives of virology2025

Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents.

Mantong Zhu, Chang Liu, Guangfeng Liu, Hongsai Zhang, Xing Wang, Ying Wang, Yongqi Luo, Jingzhe Jiang

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mantong Zhu *Dalian Ocean University, Dalian, China.
Chang Liu *Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China.
Guangfeng LiuKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China.
Hongsai ZhangKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China.
Xing WangKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China.
Ying WangKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China.
Yongqi LuoKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China.
Jingzhe JiangKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China. jingzhejiang@gmail.com.ORCID http://orcid.org/0000-0001-5260-7822

Funding

Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences 2022TS05Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences 2023TD44the Hainan Provincial Natural Science Foundation of China 324CXTD435the Key-Area Research and Development Program of Guangdong Province 2022B1111030001
6 · The paper itself

Abstract

A Providencia strain was isolated from farm effluents and used as a host to isolate a bacteriophage named A06. A double-layer agar plaque assay was used to determine the optimal multiplicity of infection (MOI), construct a one-step growth curve, evaluate the temperature and pH stability of the phage, and assess its in vitro antibacterial activity and lysis efficiency. Whole-genome sequencing, functional annotation, and comparative genomics analysis were used to analyze its biological features and determine its taxonomic position. Bacteriophage A06 has a circular DNA genome with 58 open reading frames (ORFs). The optimal MOI was determined to be 100, and a one-step growth curve showed the latent period to be 40 minutes and the lysis period to range from 40 to 90 minutes. Bacteriophage A06 exhibited a high degree of thermal stability and pH tolerance, and genomic analysis indicated that it represents a new species within the genus Kakivirus of the family Autoscriptoviridae.

Indexed as

BacteriophagesFarmsProvidenciaWaste ProductsEnterobacteriaceae InfectionsGenome, ViralPhage TherapyPhylogenyRNA, Ribosomal, 16SRNA, Ribosomal, 16SWaste Products

Identifiers

PMID40731076

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.