ArticleNature microbiology2025
Peptidoglycan-outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria.
Article in Nature microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- LPS in gram-negative bacterial growth and physiology: outside but fully integrated.Microbiology and molecular biology reviews : MMBR · 2026Review
- Independent tuning of outer membrane fluidity and mechanics in Gram-negative bacteria.bioRxiv : the preprint server for biology · 2026Article
- Antibiotic Resistance and the Return to a Pre-Antibiotic Era: A Critical Narrative Review of a Global Catastrophe.Biomedicines · 2026Review
- Stress-induced membrane insertion at the β-barrel assembly machinery complex regulates BepA metalloprotease activity.Nature communications · 2026Article
- Peptidoglycan Remodeling in Gram-Negative Bacteria: From Stress Adaptation to Antibiotic Tolerance and Therapeutic Targeting.Antibiotics (Basel, Switzerland) · 2026Review
- Quantifying outer membrane permeability to β-lactam antibiotics using outer membrane vesicles that protect antibiotic-susceptible bacteria.Nature communications · 2026Article
- Structure and function of Salmonella inner membrane.Archives of microbiology · 2026Review
- Outer Membrane-Peptidoglycan Anchoring inJournal of the American Chemical Society · 2026Article
- A periplasmic layer formed by an outer membrane lipoprotein governs the cell-envelope integrity and stiffness of Leptospira interrogans.Communications biology · 2026Article
- Stress-Driven Accelerated Evolution and Ecological Network Reconfiguration in Extremophilic Microbial Communities.Biology · 2026Review
- Peptidoglycan remodeling improves salt resilience ofApplied and environmental microbiology · 2026Article
- Enhanced nanoencapsulation of gentamicin, ciprofloxacin, and lysozyme in chitosan-MIL-53 metal-organic frameworks for a synergistic activity against drug-resistantRSC advances · 2026Article
- Article
- A potential role for acyl-phosphate in the coordination of phospholipid and lipopolysaccharide synthesis inbioRxiv : the preprint server for biology · 2026Article
- Bacteriocin biophysics: From protein-protein interaction models to navigators of the bacterial cell envelope.The Journal of biological chemistry · 2026Article
- Selective elimination of donor bacteria to analyze plasmid reactions during conjugative transfer.Biology methods & protocols · 2026Review
- Peptidoglycan LD-Transpeptidases.Antibiotics (Basel, Switzerland) · 2025Review
- Mechanisms of Pseudomonas aeruginosa resistance to type VI secretion system attacks.Nature communications · 2025Article
- Selective elimination of donor bacteria enables global profiling of plasmid gene expression at early stages of conjugation.Nucleic acids research · 2025Article
- Integrated Phenotypic and Transcriptomic Analyses Unveil the Antibacterial Mechanism of Punicalagin Against Methicillin-ResistantFoods (Basel, Switzerland) · 2025Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Bacteria are subject to a substantial concentration differential of osmolytes between the interior and exterior of the cell, resulting in turgor pressure. Failure to mechanically balance this turgor pressure causes cells to burst. Here, using microfluidics, imaging, biochemistry and mathematical modelling, we analysed how Escherichia coli cells with structural mutations in the envelope respond to hypoosmotic shocks. We show that the peptidoglycan cell wall forms a mechanical unit with the outer membrane that limits periplasmic volume increase under hypoosmotic shock, allowing osmotic pressure build-up in the periplasm. In turn, this periplasmic pressure balances cytoplasmic turgor across the inner membrane, preventing cell lysis and death. Thus, while the peptidoglycan layer is necessary, it is not sufficient to maintain turgor and protect cells from lysis. We propose a model in which the entire cell envelope, including the periplasm, collectively enables Gram-negative bacteria to overcome osmotic challenges.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.