ArticlePlant cell reports2025
Agrobacterium-mediated transformation for virus-induced gene silencing (VIGS) in Castilleja tenuiflora: Cte-chlH and Cte-PDS.
Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Establishment of a TRV-based virus-induced gene silencing system using the vacuum infiltration in Tartary buckwheat.Plant cell reports · 2025Article
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5 authors.
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Abstract
key messageAgrobacterium-mediated transformation allowed the establishment of the VIGS system based on the TRV for specific marker genes, Cte-PDS and Cte-chlH, in C. tenuiflora, a hemiparasitic plant. Castilleja tenuiflora Benth. (Orobanchaceae) is a medicinal and hemiparasitic plant recognized for its specialized metabolism. Genetic transformation systems are valuable for gene function analysis and understanding the regulation of the biosynthetic pathways of bioactive molecules. Here, we present two efficient Agrobacterium-mediated transformation protocols, defined as "transformation by injection" (Ti-AI) and "transformation by cocultivation" (Tc-AII). For both methods, two strains of A. tumefaciens were used: PCH32 and C58C1. These strains harbored a binary expression vector containing β-glucuronidase (GUS) as a reporter gene. The putative transformants tested positive in the GUS histochemical staining assay, which was confirmed via PCR. Tc-AII showed a higher transformation efficiency (Ti-AI: 23 vs. Tc-AII: 65%) although Ti-AI generated the highest survival rate of the putative transformants (Ti-AI: 75 vs. Tc-AII: 37%). Tc-AII-C58C1 was used to evaluate a VIGS system based on pTRV. Vectors were constructed with phytoene desaturase (Cte-PDS) and protoporphyrin magnesium chelatase subunit H (Cte-chlH) genes. Successful VIGS was demonstrated by gene silencing observed across various growth stages of C. tenuiflora when pTRV
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