Evidence map›Paper›PMID 40730576›Full record

ArticleNPJ science of food2025

Rhizobium biostimulation of blackberry modulates survival pathways in Caenorhabditis elegans across biological kingdoms.

Rocío Roca-Couso, José David Flores-Félix, Begoña Ayuda-Durán, Rebeca Ferreras-Charro, Ignacio García-Estévez, Paula García-Fraile, Raúl Rivas

Abstract read
In one paragraph

Article in NPJ science of food, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Mechanisms of Anti-Aging Effect ofMolecules (Basel, Switzerland) · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rocío Roca-CousoDepartment of Microbiology and Genetics, Edificio Departamental de Biología, Universidad de Salamanca, Salamanca, Spain.
José David Flores-FélixDepartment of Microbiology and Genetics, Edificio Departamental de Biología, Universidad de Salamanca, Salamanca, Spain. jdflores@usal.es.
Begoña Ayuda-DuránDepartment of Analytical Chemistry, Nutrition and Food Science, Universidad de Salamanca, Salamanca, Spain.
Rebeca Ferreras-CharroDepartment of Analytical Chemistry, Nutrition and Food Science, Universidad de Salamanca, Salamanca, Spain.
Ignacio García-EstévezDepartment of Analytical Chemistry, Nutrition and Food Science, Universidad de Salamanca, Salamanca, Spain.
Paula García-FraileDepartment of Microbiology and Genetics, Edificio Departamental de Biología, Universidad de Salamanca, Salamanca, Spain.
Raúl RivasDepartment of Microbiology and Genetics, Edificio Departamental de Biología, Universidad de Salamanca, Salamanca, Spain.

Funding

European Commission 101034371Ministerio de Ciencia e Innovación PRE2020-094762
6 · The paper itself

Abstract

Endophytic Rhizobium species represent promising bioinoculants for enhancing crop performance and nutritional profiles. This study investigated the impact of Rhizobium sp. CRRU65 inoculation on blackberry (Rubus sp.) plants, with emphasis on fruit phytochemical composition and cross-kingdom bioactivity. Inoculated plants exhibited a significant increase in yield and elevated levels of phenolic compounds, notably sanguiin H6 and cyanidin-3-O-glucoside, as quantified by HPLC-DAD-MS. Antioxidant functionality was evaluated using Caenorhabditis elegans under oxidative stress. Extracts from inoculated fruits significantly improved nematode survival, accompanied by transcriptional upregulation of skn-1 and hsp-16, genes involved in stress response and proteostasis. These findings demonstrate that Rhizobium sp. CRRU65 enhances not only agronomic traits but also the nutraceutical quality of blackberry fruits, with beneficial effects extending across biological kingdoms. This work underscores the potential of endophytic bacteria to contribute to sustainable agriculture and functional food innovation through molecular and physiological modulation in both plants and animal models.

Identifiers

PMID40730576
PMCPMC12307951

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.