Evidence map›Paper›PMID 40730299›Full record

ArticleJournal of advanced research2026

Harnessing GhGRDP1 natural variation for enhanced cotton seed yield: a rhamnose-dependent strategy in breeding.

Yongyan Zhao, Hongyu Wu, Ting Zhao, Shengcai Huang, Yupeng Hao, Yidan Chen, Shouli Feng, Luyao Wang, Yumeng Zhu, Kai Huang and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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0cells of the map it votes in
2citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Yongyan ZhaoThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China; Hainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China.
Hongyu WuThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China.
Ting ZhaoThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China; Hainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China.
Shengcai HuangThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China.
Yupeng HaoThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China.
Yidan ChenCollege of Life Sciences, Nanjing Normal University, Nanjing, China.
Shouli FengXianghu Laboratory, Hangzhou 311231, China.
Luyao WangHainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China.
Yumeng ZhuThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China; Hainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China.
Kai HuangThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China; Hainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China.
Jin HanThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China.
Yiqian LiThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China.
Siyuan WangThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China.
Zeyu DongThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China.
Tianneng ZhuInstitute of Bioinformatics, Zhejiang University, Hangzhou, Zhejiang Province, China.
Shengjun ZhaoThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China; Hainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China.
Yajun LiangXinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production/Cotton Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumuqi 830091 Xinjiang, China.
Zhiyuan ZhangHainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China.
Bojian ZhongCollege of Life Sciences, Nanjing Normal University, Nanjing, China.
Hai SongDepartment of Thoracic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Tianzhen ZhangThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China; Hainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China; Key Laboratory of Speed Breeding in Plant Factory, Ministry of Agriculture and Rural Affairs, China.
Xueying GuanThe Advanced Seed Institute, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, Plant Precision Breeding Academy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 300058, China; Hainan Institute of Zhejiang University, Building 11, Yongyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya, Hainan 572025, China; Key Laboratory of Speed Breeding in Plant Factory, Ministry of Agriculture and Rural Affairs, China; Xinjiang Agricultural University, Urumqi, Xinjiang, China. Electronic address: xueyingguan@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionBreeding high-yield crops has long been a fundamental goal in global agriculture, with seeds serving as the essential vehicle to achieve this aim. Therefore, understanding the regulatory mechanisms of seed size is critical for ensuring food security.

objectivesOur study focused on the natural variation, molecular regulatory mechanism and functional evolution of yield trait-related gene GhGRDP1, identified through GWAS and eQTL analyses in upland cotton natural population.

methodsPopulation resequencing and phenotypic data analysis were combined with Kompetitive Allele Specific PCR, degradome, GUS staining assays, and qPCR revealed the natural variation, evolutionary history and geographic origins of GhGRDP1. Using CRISPR-Cas9 gene editing and plant transformation techniques, GRDP1 transgenic plants of cotton, rice and Arabidopsis were generated to unravel its conserved role in seed regulation. Co-IP, pull down, BiFC, LCI and enzyme activity assays were used to explore the molecular mechanism of GhGRDP1. The evolution of GRDP1 was understood through phylogenetic analysis and protein structure prediction, segmental interaction and enzyme activity assays.

resultsA 1-bp deletion in the eighth exon of GhGRDP1 initiates the nonsense-mediated mRNA decay (NMD) mechanism, causing variations in seed index (SI) and lint percent (LP) among cotton populations. This variant, unique to Gossypium hirsutum, originated from specific races and was strongly selected during domestication. GhGRDP1 positively regulates seed size and weight without compromising fiber quality, correlating with osmolyte accumulation in ovules. GhGRDP1 interacts with UDP-L-rhamnose synthase (GhRHM1) in vivo and in vitro, functioning as an activator of GhRHM1 enzymatic activity involved in cell wall composition. Notably, GRDP1 evolved a short glycine-rich domain (sGRD) on C-terminal throughout core angiosperms, playing a conserved role in seed development across both monocots and eudicots.

conclusionThese findings significantly advance understanding the genetic basis of phenotypic variation in cotton seed yield, providing valuable targets for high-yield crop breeding.

Indexed as

GossypiumPlant BreedingPlant ProteinsSeedsCrops, AgriculturalGene EditingGene Expression Regulation, PlantGenetic VariationPhenotypePhylogenyPlants, Genetically ModifiedQuantitative Trait LociPlant ProteinsDomesticationGRDPNonsense-mediated mRNA decaySeed yieldUDP-L-rhamnose synthesisUpland cotton (Gossypium hirsutum)

Identifiers

PMID40730299
PMCPMC13001069

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