ArticleBasic research in cardiology2025
Cold storage of mouse hearts prior to cardiomyocyte isolation preserves electromechanical function, microstructure, and gene expression for 24 h.
Article in Basic research in cardiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Article
- Models of the human heart for biomedical research: Opportunities and challenges.Physiological reports · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Isolation of myocytes from mouse hearts, especially of transgenic animals or disease models, is crucial in cardiac research. The presumption that cardiomyocytes must be isolated immediately after heart procurement to avoid deterioration implies that transgenic mouse lines must be present on site, causes schedule inflexibility, and hampers collaborations, thereby increasing the number, suffering, and costs of animals. This study challenges this presumption by investigating whether the cell isolation can be postponed for 24 h without affecting the results. Adult mouse hearts were subjected to enzymatic myocyte isolation immediately after excision (CTRL) or after 24 h of cold storage (CS). Sufficient numbers of viable cardiomyocytes were obtained in all groups. The transverse-axial tubular system was unchanged in CS versus CTRL. No significant changes were detected in cell capacitance, resting membrane potential, action potential shape and duration, amplitudes, and kinetics of the K
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.