Evidence map›Paper›PMID 40728720›Full record

ArticleInternational urogynecology journal2025

Gynecologic Oncology Patients have Urobiomes that are Similar to Women Without Gynecologic Cancer.

Anisa Nasse, Abigail Winder, Melline Fontes Noronha, Margaret R Liotta, Ronald K Potkul, Elizabeth R Mueller, Linda Brubaker, Alan J Wolfe

Abstract read
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In one paragraph

Article in International urogynecology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anisa NasseDepartment of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA.
Abigail WinderDepartment of Obstetrics and Gynecology, Loyola University Medical Center, Maywood, IL, 60130, USA.
Melline Fontes NoronhaDepartment of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA.
Margaret R LiottaDepartment of Obstetrics and Gynecology, Loyola University Medical Center, Maywood, IL, 60130, USA.
Ronald K PotkulDepartment of Obstetrics and Gynecology, Loyola University Medical Center, Maywood, IL, 60130, USA.
Elizabeth R MuellerDepartment of Obstetrics and Gynecology, Loyola University Medical Center, Maywood, IL, 60130, USA.
Linda BrubakerDepartment of Obstetrics and Gynecology, Loyola University Medical Center, Maywood, IL, 60130, USA. librubaker@health.ucsd.edu.
Alan J WolfeDepartment of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA. awolfe@luc.edu.ORCID 0000-0003-4532-0545

Funding

The Female Urinary Microbiome and Urinary IncontinenceR01DK104718 · NIDDK · LOYOLA UNIVERSITY CHICAGO · PI BRUBAKER, LINDA, WOLFE, ALAN J · 2016 to 2020
$3.1M
The Female Urinary Microbiome: Adjacent Microbiomes and Clinical AssociationsP20DK108268 · NIDDK · LOYOLA UNIVERSITY CHICAGO · PI WOLFE, ALAN J · 2015 to 2016
$583k
Urinary Bacterial Communities in Urge Incontinence WomenR21DK097435 · NIDDK · LOYOLA UNIVERSITY CHICAGO · PI BRUBAKER, LINDA, GAI, XIAOWU · 2013 to 2014
$414k
The Female Urinary Microbiome and Urinary IncontinenceR56DK104718 · NIDDK · LOYOLA UNIVERSITY CHICAGO · PI BRUBAKER, LINDA, WOLFE, ALAN J · 2015 to 2015
$300k
Falk Foundation LU#202567NIDDK NIH HHS P20 DK108268NIDDK NIH HHS R01 DK104718NIDDK NIH HHS R21 DK097435NIDDK NIH HHS R56 DK104718
6 · The paper itself

Abstract

introduction and hypothesisTo compare the bladder microbiomes of participants with and without a gynecological cancer at the time of diagnostic surgery to determine their suitability as control participants in urobiome research studies.

methodsIRB-approved clinical sample of continent adult female patients undergoing surgery for suspected gynecologic malignancy. Participants contributed clinical data (abstracted from the electronic medical record), and a catheterized urine sample obtained prior to antibiotic administration on the day of surgery. The study urine sample underwent DNA sequencing following amplification of hyper-variable region 4 (V4) of the bacterial 16S rRNA gene. The sequences were processed, annotated, and decontaminated. Alpha (within-sample) and beta (between-sample) diversity indices were computed, and the microbiome compositions across all participants were compared.

resultsNinety-two women participated, including 20 with a gynecological cancer diagnosis and a comparison group of 72 without gynecologic cancer detected. The mean age was 53 years old (range 22-92) with a mean BMI of 31 kg/m

conclusionsParticipants with a gynecological cancer diagnosis can be used as controls for bladder microbiome studies.

Indexed as

Genital Neoplasms, FemaleMicrobiotaUrinary BladderAdultAgedAged, 80 and overCase-Control StudiesFemaleHumansMiddle AgedRNA, Ribosomal, 16SYoung AdultRNA, Ribosomal, 16S16S rRNA gene sequencingBladderGynecological cancerMicrobiomeUrinary tract infectionUrobiomeUrogynecology

Identifiers

PMID40728720

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.