Evidence map›Paper›PMID 40727711›Full record

ArticleIntegrative medicine research2025

Mast cell degranulation mediates analgesic effects of acupuncture at different stimulation durations in arthritic rats.

Yuhang Liu, Mingzhu Sun, Yixuan Wang, Yingchen Li, Ziliang Zhang, Liujie Ren, Wei Yao, Yi Yu

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In one paragraph

Article in Integrative medicine research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yuhang LiuShanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, College of Biomedical Engineering, Fudan University, Shanghai, China.
Mingzhu SunShanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, College of Biomedical Engineering, Fudan University, Shanghai, China.
Yixuan WangShanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, College of Biomedical Engineering, Fudan University, Shanghai, China.
Yingchen LiShanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, College of Biomedical Engineering, Fudan University, Shanghai, China.
Ziliang ZhangShanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, College of Biomedical Engineering, Fudan University, Shanghai, China.
Liujie RenFPRS Department, Eye & ENT Hospital of Fudan University, Shanghai, China.
Wei YaoShanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, College of Biomedical Engineering, Fudan University, Shanghai, China.
Yi YuCollege of Medical Instruments, Shanghai University of Medicine & Health Sciences, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: To understand the effect and mechanism of different acupuncture durations on analgesic effects. Methods: An adjuvant-induced arthritis rat model evaluated the therapeutic effects of acupuncture at the Zusanli acupoint (ST36). A robotic arm provided stable lifting-thrusting stimulation for different durations (2, 5, 10, 20, and 30 min). Mechanical and thermal pain thresholds were measured before and after treatment to assess the analgesic effects. Additionally, tissue sections from ST36 were stained and analyzed to assess mast cell degranulation rates, and in vitro stretching experiments were performed at different stretching times (0-60 min) to measure the release of histamine, 5-hydroxytryptamine, and leukotrienes. Results: Within the 30 min acupuncture duration range set in this study, the pain threshold recovery rate in rats exhibited a steady upward trend as the acupuncture duration gradually increased, reaching its optimal level between 20 and 30 min. The mast cell degranulation rate exhibited a similar time-related trend, reaching 80-90 % at 20-30 min. Correlation analysis revealed a significant positive correlation between the pain threshold recovery rate and mast cell degranulation rate. In the cell-stretching experiment, the release of biochemical mediators was time-dependent. Conclusions: In the adjuvant-induced arthritis rat model, the analgesic effect observed at 20-30 min was superior to that observed at shorter durations, which was associated with the mast cell degranulation rate and release of biochemical mediators in the tissue.

Indexed as

Acupuncture durationAdjuvant-induced rat modelAnalgesiaMast cellPain threshold

Identifiers

PMID40727711
PMCPMC12302654

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.