ArticleJournal of biomedical optics2025
Three-dimensional scanless patterned illumination using time-multiplexed multiline temporal focusing for multicell manipulation with single-cell resolution.
Article in Journal of biomedical optics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
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Abstract
Significance: Three-dimensional (3D) two-photon patterned illumination using a combination of computer-generated holography (CGH) and wide-field temporal focusing (TF) has emerged as a highly effective approach for photostimulation. However, even though the axial full-width at half-maximum of a single-spot by TF is smaller than the single-cell size of Aim: We aim to achieve 3D two-photon patterned illumination with single-cell resolution by combining CGH with time-multiplexed multiline temporal focusing (TM-ML-TF), which is implemented by adding an echelle grating at a position conjugate to the focal plane of the TF-CGH system. Approach: We measure the 3D two-photon fluorescence distributions generated by the TF-CGH and TM-ML-TF-CGH systems. Results: In TM-ML-TF, the crosstalk artifacts between the target spots in two proximal planes with an axial distance of Conclusions: TM-ML-TF successfully suppresses the interference among multispots, enabling the TM-ML-TF-CGH system to provide precise 3D patterned illumination with single-cell resolution.
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