ArticleAnalytical chemistry2025
Single-Cell 5 μm-Resolution Dual-Polarity MALDI-MS Imaging without Matrix Reapplication.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- MALDI mass spectrometry imaging across spatial scales in toxicology: tissue evidence, cellular heterogeneity, and emerging single-cell opportunities.Analytical and bioanalytical chemistry · 2026Review
- Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.Analytical and bioanalytical chemistry · 2026Review
- Tissue expansion mass spectrometry imaging (TEMI) for high-spatial-resolution multiomics molecular mapping.Nature protocols · 2026Review
- MALDI Mass Spectrometry Imaging in Alzheimer's Disease Lipidomics: Matrix Selection, Spatial Lipid Pathology and Emerging Analytical Strategies.International journal of molecular sciences · 2026Review
- Protocol for reconstructing 2D and 3D peptide atlases of the Ciona cerebral ganglion at single-cell resolution.STAR protocols · 2026Article
- Feature Down-Selection to Improve Supervised Classification by Machine Learning on Mass Spectrometry Imaging Data.Molecules (Basel, Switzerland) · 2026Article
- SMASH Imaging: A Serial Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Strategy for High-Resolution Imaging Facilitates Dual-Polarity and MS2 Spatial Lipidomics on a Single Tissue Section.Analytical chemistry · 2026Article
- Single-cell metabolite annotation by tandem mass spectrometry imaging andRSC advances · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution mass spectrometry imaging (MSI) plays a vital role in lipidomics, yet challenges persist in analyzing lipids at the single-cell level due to limitations in spatial resolution and lipid coverage. While existing strategies based on a single matrix application step for dual-polarity provide high lipid coverage from the same sample and enable easy sample preparation, matrix depletion limits their spatial resolution to 10 μm, preventing their application to single-cell imaging. Here, we present a single-cell/subcellular resolution strategy for dual-polarity matrix-assisted laser desorption and ionization mass spectrometry imaging (MALDI-MSI) that eliminates the need for matrix reapplication. This approach achieves 5 μm spatial resolution while maintaining lipid coverage comparable to multistep single-cell imaging methods. This is enabled by a fine-tuned matrix deposition technique that fully utilizes the high sensitivity of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.