ArticleJournal of cell science2025
Cell volume regulates terminal differentiation of cultured human epidermal keratinocytes.
Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- YAP1-dependent regulation of cell size in BCAM-positive limbal corneal progenitor cells.Experimental eye research · 2026Article
- Micropillar Topography Regulates Morphology and Melanogenesis in Melanoma Cells.Journal of functional biomaterials · 2026Article
- In Vitro Model Characterizing Carcinogenic Progression of HPV-Positive Oropharyngeal Cancer.Cancers · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
To gain insights into the human epidermal stem cell niche, we have previously identified micron-scale topographical substrates that regulate differentiation of spread keratinocytes. On one substrate (S1), cells interact with circular topographies and differentiation is stimulated; on the other (S2), cells interact with triangular topographies and differentiation is inhibited. Cell stiffness on S1 and S2 was similar, and nuclear localisation of the mechano-sensitive transcriptional regulator YAP1 was decreased on S1 and S2 compared to on flat substrates. However, cells on S2 exhibited reduced cell volume, leading us to explore the potential for volume-regulated differentiation. Treatment with polyethylene glycol decreased cell volume and inhibited differentiation under a range of conditions. Conversely, deionized water increased cell volume and stimulated differentiation. Bulk RNA sequencing identified several substrate-responsive genes, including aquaporins and ion channels. A membrane permeable Ca2+ chelator and an inhibitor of the water channel aquaporin 3 blocked volume-induced differentiation. These studies identify cell volume as a mechanism by which keratinocyte-niche interactions regulate terminal differentiation.
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Registered trials
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