Evidence map›Paper›PMID 40725407›Full record

ArticleGenes2025

Aluminum Stress Response Is Regulated Through a miR156/SPL13 Module in

Gamalat Allam, Solihu K Sakariyahu, Binghui Shan, Banyar Aung, Tim McDowell, Yousef Papadopoulos, Mark A Bernards, Abdelali Hannoufa

Abstract read
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In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Gamalat AllamAgriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.ORCID 0009-0009-4929-7101
Solihu K SakariyahuAgriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.ORCID 0000-0002-5130-6662
Binghui ShanAgriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.
Banyar AungDepartment of Biology, University of Western Ontario, 1151 Richmond Street, London, ON N6A 3K7, Canada.
Tim McDowellAgriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.ORCID 0000-0001-5334-1923
Yousef PapadopoulosAgriculture and Agri-Food Canada, 58 River Road, Truro, NS B2N 5E3, Canada.
Mark A BernardsDepartment of Biology, University of Western Ontario, 1151 Richmond Street, London, ON N6A 3K7, Canada.ORCID 0000-0003-1209-3200
Abdelali HannoufaAgriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.ORCID 0000-0003-0064-620X

Funding

The Natural Sciences and Engineering Research Council of Canada RGPIN-2018-04241
6 · The paper itself

Abstract

backgroundAluminum (Al) toxicity severely limits

methodsGene expression analyses, histochemical staining, nutrient profiling, phenotypic assays, transcriptome profiling, and ChIP-seq were conducted on alfalfa plants with altered miR156 and SPL13 expression to assess their roles in the Al stress response.

resultsAl stress induced SPL13 expression while repressing miR156 in the roots. Elevated miR156 intensified Al accumulation, lipid peroxidation, and plasma membrane damage, accompanied by reduced leaf nitrogen, magnesium, sulfur, and phosphorus content. Phenotypically, increased SPL13 enhanced the root length and Al tolerance, whereas SPL13 silencing reduced tolerance. Transcriptome profiling of SPL13-silenced plants identified differentially expressed genes involved in the Al response, including aluminum-activated malate transporters and various transcription factors (GRAS, Myb-related, bHLH041, NAC, WRKY53, bZIP, and MADS-box). ChIP-seq revealed that SPL13 directly regulates genes encoding a protein kinase, cytochrome P450, and fasciclin-like arabinogalactan proteins.

conclusionsThe MsmiR156/MsSPL13 network plays a crucial regulatory role in alfalfa's response to Al toxicity. These findings provide novel genetic targets and foundational knowledge to advance molecular breeding for enhanced Al tolerance in alfalfa.

Indexed as

AluminumMedicago sativaMicroRNAsPlant ProteinsStress, PhysiologicalTranscription FactorsGene Expression ProfilingGene Expression Regulation, PlantPlant RootsAluminumMicroRNAsPlant ProteinsTranscription FactorsAl toxicityChIP-seqMedicago sativamiR156root developmentSPL13transcriptome profiling

Identifiers

PMID40725407
PMCPMC12295421

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.