Evidence map›Paper›PMID 40725108›Full record

ArticleInternational journal of molecular sciences2025

Detection of Feline Coronavirus Membrane Gene Based on Conventional Revere Transcription-Polymerase Chain Reaction, Nested Reverse Transcription-Polymerase Chain Reaction, and Reverse Transcription-Quantitative Polymerase Chain Reaction: A Comparative Study.

Chiraphat Kopduang, Witsanu Rapichai, Chalandhorn Leangcharoenpong, Piyamat Khamsingnok, Thanapol Puangmalee, Siriluk Ratanabunyong, Amonpun Rattanasrisomporn, Thanawat Khaoiam, Hieu Van Dong, Kiattawee Choowongkomol and 1 more

Abstract readComparative Study
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chiraphat KopduangGraduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.ORCID 0009-0005-7030-8953
Witsanu RapichaiDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.ORCID 0009-0001-8529-4137
Chalandhorn LeangcharoenpongGraduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.ORCID 0009-0006-5066-0041
Piyamat KhamsingnokGraduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Thanapol PuangmaleeGraduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Siriluk RatanabunyongDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0003-1078-186X
Amonpun RattanasrisompornInterdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, Thailand.
Thanawat KhaoiamDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Hieu Van DongDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0002-6340-9204
Kiattawee ChoowongkomolDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0002-2421-7859
Jatuporn RattanasrisompornGraduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.ORCID 0000-0002-2426-0961

Funding

The research was funded by the Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
6 · The paper itself

Abstract

Feline coronavirus (FCoV) is a major pathogen causing feline infectious peritonitis (FIP), a lethal disease in cats, necessitating accurate diagnostic methods. This study developed and compared novel primers targeting the FCoV membrane (

Indexed as

Coronavirus, FelineFeline Infectious PeritonitisReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionAnimalsCatsDNA PrimersRNA, ViralSensitivity and SpecificityDNA PrimersRNA, Viralfeline coronavirusFIPnested RT-PCRRT-PCRRT-qPCR

Identifiers

PMID40725108
PMCPMC12295316

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.