Evidence map›Paper›PMID 40724345›Full record

ArticleFoods (Basel, Switzerland)2025

Rapid Kinetic Fluorogenic Quantification of Malondialdehyde in Ground Beef.

Keshav Raj Bhandari, Max Wamsley, Bindu Nanduri, Willard E Collier, Dongmao Zhang

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Keshav Raj BhandariDepartment of Chemistry, Mississippi State University, Starkville, MS 39762, USA.ORCID 0000-0001-6988-0753
Max WamsleyDepartment of Chemistry, Mississippi State University, Starkville, MS 39762, USA.
Bindu NanduriDepartment of Basic sciences, Mississippi State University, Starkville, MS 39762, USA.
Willard E CollierDepartment of Chemistry, Tuskegee University, Tuskegee, AL 36088, USA.ORCID 0009-0003-8683-5972
Dongmao ZhangDepartment of Chemistry, Mississippi State University, Starkville, MS 39762, USA.

Funding

Zinc dependent conformational changes in AdcR and their impact on DNA bindingP20GM103646 · NIGMS · MISSISSIPPI STATE UNIVERSITY · PI THIBAUDEAU, GISELLE · 2013 to 2022
$21.1M
NIGMS NIH HHS P20 GM103646NSF AND NIGMS CHE 2203571 AND P20GM103646
6 · The paper itself

Abstract

Malondialdehyde (MDA), a mutagenic and carcinogenic compound, is widely studied in the meat industry and lipid peroxidation research due to its implications for food quality and safety. Current methods for quantifying MDA in solid tissues are labor-intensive, requiring multiple instruments and approximately two hours to complete. This study presents an ultrafast kinetic fluorogenic method for quantifying MDA in ground beef, utilizing 2-thiobarbituric acid (TBA) as a fluorogenic probe. The total assay time is significantly shortened to 6 min from sample preparation to data acquisition. The assay's robustness against matrix interference was validated using sample volume variation and standard addition calibration methods. Additionally, the effects of ambient exposure to air, washing, and cooking on MDA content in raw ground beef were quantified. While both ambient exposure to air and cooking increased MDA levels, washing raw ground beef and decanting cooked ground beef broth effectively reduced MDA levels in the ground beef. This simple and rapid assay can be adopted both in food research and industry. Moreover, insights from our study on the relationship between ground beef treatment and MDA concentration will help consumers make informed decisions about ground beef handling and consumption to lower their intake of MDA.

Indexed as

fluorogenicground beefkinetic fluorescencelipid oxidationmalondialdehyde

Identifiers

PMID40724345
PMCPMC12295034

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.