Evidence map›Paper›PMID 40723816›Full record

ArticleBiomolecules2025

Probing the Active Site of Class 3 L-Asparaginase by Mutagenesis: Mutations of the Ser-Lys Tandems of ReAV.

Kinga Pokrywka, Marta Grzechowiak, Joanna Sliwiak, Paulina Worsztynowicz, Joanna I Loch, Milosz Ruszkowski, Miroslaw Gilski, Mariusz Jaskolski

Abstract read
In one paragraph

Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kinga PokrywkaInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Marta GrzechowiakInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.ORCID 0000-0002-7605-1563
Joanna SliwiakInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.ORCID 0000-0003-1153-6380
Paulina WorsztynowiczInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Joanna I LochDepartment of Crystal Chemistry and Crystal Physics, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland.ORCID 0000-0002-7345-4527
Milosz RuszkowskiInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.ORCID 0000-0002-4415-3100
Miroslaw GilskiInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.ORCID 0000-0002-3941-9947
Mariusz JaskolskiInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.ORCID 0000-0003-1587-6489

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ReAV enzyme from

Indexed as

AsparaginaseBacterial ProteinsAmino Acid SequenceBinding SitesCatalytic DomainCrystallography, X-RayLysineModels, MolecularMutagenesis, Site-DirectedMutationSerineAsparaginaseBacterial ProteinsLysineSerineamidohydrolasehydrolaseITCL-asparaginaseleukemiametalloproteinNessler reactionsite-directed mutagenesisX-ray crystallography

Identifiers

PMID40723816
PMCPMC12292245

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.