ArticleBioengineering (Basel, Switzerland)2025
Do Preparation Techniques Transform the Metabolite Profile of Platelet-Rich Plasma?
Article in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Multivariate Temporal Inflammatory-Regenerative Signatures of Bovine Platelet-Rich Gel Supernatants Under Different Storage Temperatures.Gels (Basel, Switzerland) · 2026Article
- From Regenerative Mechanisms to Clinical Practice: Current Status, Controversies, and Future Perspectives of Platelet-Rich Plasma in Urology and Sexual Medicine.Journal of clinical medicine · 2026Review
- Activated platelet rich plasma modulates the proliferation, apoptosis and matrix synthesis driven by growth factors in osteoarthritic chondrocytes: anFrontiers in bioengineering and biotechnology · 2026Article
- Recent Advancements in Non-surgical Approaches for the Management of Delayed Union and Nonunion in Long Bone Fractures of the Extremities: A Review.Advances in therapy · 2026Review
- The Platelet-Rich Plasma research ecosystem: From mechanistic insights to precision treatment in knee osteoarthritis.Regenerative therapy · 2025Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundPlatelet-rich plasma (PRP) is a widely used therapeutic product in musculoskeletal treatments due to its regenerative and anti-inflammatory properties. However, the lack of standardization in PRP preparation protocols hampers clinical consistency.
methodsIn this study, the metabolic profiles of 10 different PRP types were compared using untargeted metabolomics via Q-TOF LC-MS. PRP-G and PRP-S were prepared from six donors to assess inter-individual variability, while the remaining types were obtained from a single donor to isolate the impact of preparation method alone. Multivariate analyses, VIP scores, and pathway enrichment analyses were conducted.
resultsPRP formulations exhibited distinct metabolic differences associated with inflammatory signaling, redox homeostasis, steroid metabolism, energy production, and platelet activation. Samples from both single- and multi-donor groups showed high intra-group similarity, indicating that preparation method is a major determinant of PRP's biochemical composition.
conclusionMetabolomic profiling reveals that even minor differences in PRP preparation protocols can lead to significant biochemical changes that may affect therapeutic outcomes. This study highlights the need for standardized, indication-specific PRP products and underscores the value of metabolomic analysis in guiding optimal formulation selection in clinical practice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.