ArticleMilitary Medical Research2025
Accelerating cartilage regeneration with DNA-SF hydrogel sustained release system-based cartilage organoids.
Article in Military Medical Research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- An AI-integrated organoid platform enables high-throughput functional evaluation of bioactive metal ions.Bioactive materials · 2026Article
- Liquid-Responsive Shape-Memory Nanofiber-Reinforced Scaffolds for Cartilage Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Topographical design principles for osteochondral tissue engineering.Bioactive materials · 2026Review
- A‑Raf: A serine/threonine protein kinase with important biological functions (Review).Molecular medicine reports · 2026Review
- Emerging engineering strategies in bone organoids: From biomimetic scaffolds to dynamic microenvironmental stimulation.Bioactive materials · 2026Review
- A Fully Defined GelMA-Based Matrix Allows Fine Tuning of Tissue-Relevant Biomechanical and Biochemical Cues for Organoid Culture.Advanced healthcare materials · 2026Article
- Bioengineering DNA-based hydrogels for regenerative medicine: A review of programmable design, chemical synthesis and therapeutic potential.Materials today. Bio · 2026Review
- An Ultrasound-Responsive Bio-Adhesive Piezoelectric Hydrogel for Osteoarthritis Cartilage.Gels (Basel, Switzerland) · 2026Article
- Exploring cartilage development and disease models: applications of cartilage organoids.Inflammation and regeneration · 2026Review
- Biophysical signal-driven scaffold design for stem cell-guided osteochondral regeneration.Bioactive materials · 2026Review
- Article
- A continuous DNA repairing system for alleviating intervertebral disc degeneration.Journal of nanobiotechnology · 2026Article
- Click-chemistry hydrogel for blood vessel organoids self-sustaining delivery to enhance flap survival.Materials today. Bio · 2026Article
- A microneedle platform Co-encapsulating chondral organoids and PpIX for spatiotemporally orchestrated tumor ablation and osteochondral regeneration.Bioactive materials · 2026Article
- Organoids in Cancer Research and Regenerative Medicine: Current Status, Challenges, and Future Prospects.MedComm · 2026Review
- Poly (d, l-lactide)/polyvinyl alcohol-based injectable microspheres with inflammation alleviation and cartilage regeneration enhancement for treatment of temporomandibular joints osteoarthritis.Regenerative biomaterials · 2026Article
- Pathology-guided design of injectable hydrogels for precision therapy and cartilage regeneration in osteoarthritis.Regenerative biomaterials · 2026Review
- Organoid research: new concepts and new technologies.Burns & trauma · 2026Review
- Review
- EXO/hydrogel system for sequential regulation of endogenous hyaline cartilage regeneration.Materials today. Bio · 2025Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCartilage repair remains a considerable challenge in regenerative medicine. Despite extensive research on biomaterials for cartilage repair in recent years, issues such as prolonged repair cycles and suboptimal outcomes persist. Organoids, miniature three-dimensional (3D) tissue structures derived from the directed differentiation of stem or progenitor cells, mimic the structure and function of natural organs. Therefore, the construction of cartilage organoids (COs) holds great promise as a novel strategy for cartilage repair.
methodsThis study employed a digital light processing system to perform 3D bioprinting of a DNA-silk fibroin (DNA-SF) hydrogel sustained-release system (DSRGT) with bone-marrow mesenchymal stem cells (BMSCs) to construct millimeter-scale cerebral organoids. COs at different developmental stages were characterized, and the COs with the best cartilage phenotype were selected for in vivo cartilage repair in a rat articular cartilage defect model.
resultsThis study developed a DSRGT by covalently grafting glucosamine (which promotes cartilage matrix synthesis) and TD-198946 (which promotes chondrogenic differentiation) onto a hydrogel using acrylic acid-polyethylene glycol-N-hydroxysuccinimide (AC-PEG-NHS). In vitro, 4-week COs exhibited higher SRY-box transcription factor 9 (SOX9), type II collagen (Col II), and aggrecan (ACAN) expression and lower type I collagen (Col I) and type X collagen (Col X) expression, indicating that 4 weeks is the optimal culture duration for hyaline cartilage development. In vivo, the mitogen-activated protein kinase (MAPK) signaling pathway was upregulated in 4-week COs, enabling cartilage repair within 8 weeks. Transcriptomic analysis revealed that cartilage regenerated with 4-week COs presented gene expression profiles resembling those of healthy cartilage.
conclusionsThis study employs DSRGT to construct COs, providing an innovative strategy for the regeneration of cartilage defects.
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