ArticleMolecular biology reports2025
Mapping rat lncRNA Bdnf-as.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundLong noncoding RNAs (lncRNAs) are increasingly recognized for their roles in regulating gene expression, yet they remain poorly understood, especially in non-human species. This study investigates the lncRNA Bdnf-as in rats, which modulates the transcription of the Bdnf gene through interactions with chromatin remodelers.
methodsIn this study, we employed a variety of methodologies to identify novel antisense transcripts of Bdnf-as in the rat genome. These methodologies included step-out rapid amplification of cDNA ends, lentivirus infusion in the neonatal rat prefrontal cortex (PFC), TET-on construct expression induction with doxycycline, de novo transcriptome assembly, and bioinformatics analysis. Our findings, derived from these rigorous methods, have led to the identification of two novel antisense transcripts of Bdnf-as in the rat genome.
resultsThese transcripts, located downstream of the Bdnf coding region, exhibit splicing and appear to be influenced by overexpression of proBDNF. The application of reverse transcription from gene-specific primers in conjunction with quantitative polymerase chain reaction (qPCR) analysis revealed that Bdnf-as exhibited augmented expression exclusively following proBDNF expression induction from a lentiviral construct, and not in the presence of a mutated form. A bioinformatic analysis revealed the potential binding of the following proteins to this site: E2F1, VDR, SP3, ZNF354C, YY2, SPI1, RUNX1, and TBX3. A comparative genomic analysis revealed limited evolutionary conservation of Bdnf-as between rats, humans, and mice, reflecting the rapid divergence of lncRNAs. The analysis of RNAseq data indicates that Bdnf-as is expressed at low levels and is likely unstable, a factor that could contribute to its detection challenges.
conclusionsThe present findings offer the initial characterization of rat Bdnf-as, its differential expression depending on expression construct, thus establishing the foundation for future studies to explore its regulatory functions and protein interactions in neurobiological processes.
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