Evidence map›Paper›PMID 40720032›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Pre-mRNA Splicing Modulation by Antisense Oligonucleotides.

Natalia N Singh, Diou Luo, Ravindra N Singh

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Natalia N SinghDepartment of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA. natalias@iastate.edu.
Diou LuoDepartment of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Ravindra N SinghDepartment of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pre-mRNA splicing, a dynamic process of intron removal and exon joining, is governed by a combinatorial control exerted by overlapping cis-elements that are unique to each exon and its flanking intronic sequences. Splicing cis-elements are usually 4- to 8-nucleotide-long linear motifs that provide binding sites for specific proteins. Pre-mRNA splicing is also influenced by secondary- and higher-order RNA structures that affect accessibility of splicing cis-elements. Antisense oligonucleotides (ASOs) that block splicing cis-elements and/or affect RNA structure have been shown to modulate splicing in vivo. Therefore, ASO-based strategies have emerged as a powerful tool for therapeutic manipulation of splicing in pathological conditions. Here we describe an ASO-based approach to increase the production of the full-length SMN2 mRNA in Spinal Muscular Atrophy patient cells.

Indexed as

Oligonucleotides, AntisenseRNA PrecursorsRNA SplicingExonsHumansIntronsMuscular Atrophy, SpinalRNA, MessengerSurvival of Motor Neuron 2 ProteinOligonucleotides, AntisenseRNA, MessengerRNA PrecursorsSMN2 protein, humanSurvival of Motor Neuron 2 Protein2′-O-methyl modificationAntisense oligonucleotide (ASO)Intronic Splicing Silencer N1 (ISS-N1)nucleofectionphosphoroamidate morpholino oligonucleotide (PMO)phosphorothioate backbonepre-mRNA splicingSpinal Muscular Atrophy (SMA)Survival Motor Neuron (SMN)transfection

Identifiers

PMID40720032

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.