Evidence map›Paper›PMID 40719977›Full record

Trial reportSports medicine (Auckland, N.Z.)2025

Erythrocyte Glycolytic and Redox Metabolism Affects Muscle Oxygenation and Exercise Performance: A Randomized Double-Blind Crossover Study in Men.

Panagiotis N Chatzinikolaou, Nikos V Margaritelis, Vassilis Paschalis, Anastasios A Theodorou, Eleni Moushi, Ioannis S Vrabas, Antonios Kyparos, Ioannis G Fatouros, Angelo D'Alessandro, Michalis G Nikolaidis

Abstract readRandomized Controlled Trial
In one paragraph

Trial report in Sports medicine (Auckland, N.Z.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Article
  4. Proteoforms as the true units of physiological function.European journal of applied physiology · 2026
    Review
  5. Erythrocytes as a Source of Exerkines.International journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Panagiotis N ChatzinikolaouDepartment of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Serres, Greece.ORCID 0000-0002-8136-1638
Nikos V MargaritelisDepartment of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Serres, Greece.
Vassilis PaschalisSchool of Physical Education and Sport Science, National and Kapodistrian University of Athens, Athens, Greece.
Anastasios A TheodorouDepartment of Life Sciences, School of Sciences, European University Cyprus, Nicosia, Cyprus.
Eleni MoushiDepartment of Life Sciences, School of Sciences, European University Cyprus, Nicosia, Cyprus.
Ioannis S VrabasDepartment of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Serres, Greece.
Antonios KyparosDepartment of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Serres, Greece.
Ioannis G FatourosDepartment of Physical Education and Sport Sciences, University of Thessaly, Trikala, Greece.
Angelo D'AlessandroDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Michalis G NikolaidisDepartment of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Serres, Greece. nikolaidis@auth.gr.ORCID 0000-0001-6165-8437

Funding

Research Committee, Aristotle University of Thessaloniki #630972
6 · The paper itself

Abstract

backgroundErythrocytes are traditionally considered passive oxygen carriers, yet their energetic and redox metabolism plays a critical role in regulating oxygen kinetics.

objectiveThis study integrates experimental and computational data to provide a comprehensive analysis of erythrocyte metabolism in response to exercise-induced oxidative stress.

methodsThe study consisted of three phases: in vivo, ex vivo, and computational. A total of 20 male participants underwent a randomized crossover experiment with two conditions: oxidative stress (eccentric contractions) and control. Oxidative stress was induced via leg eccentric contractions, and its effects on erythrocyte glycolytic and redox metabolism, arm muscle oxygenation, and arm exercise performance were evaluated. The study protocol was preregistered on the Open Science Framework ( https://osf.io/ub6zs ).

resultsEccentric contractions altered oxidative stress markers in erythrocytes (+ 22% F

conclusionIn a comprehensive exercise study investigating mechanistic relationships in erythrocyte biology, we show that erythrocyte metabolism (1) responds dynamically to exercise, (2) becomes dysregulated under oxidative stress, and (3) may partly influence muscle oxygenation and performance.

Indexed as

ErythrocytesExerciseGlycolysisMuscle, SkeletalOxidative StressOxygen ConsumptionAdultCross-Over StudiesDouble-Blind MethodHumansHydrogen PeroxideMaleOxidation-ReductionYoung AdultHydrogen Peroxide

Identifiers

PMID40719977
PMCPMC12628466

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.