Evidence map›Paper›PMID 40719898›Full record

ArticleWorld journal of microbiology & biotechnology2025

Metabolic engineering of Lactobacillus delbrueckii subsp. bulgaricus VI104 as a D-lactic acid cell factory through strategic pathway optimization for enhanced biosynthesis.

Payal Mukherjee, Senthilkumar Sivaprakasam

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Payal MukherjeeBioprocess Analytical Technology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, North Guwahati, Guwahati, Assam, 781039, India.
Senthilkumar SivaprakasamBioprocess Analytical Technology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, North Guwahati, Guwahati, Assam, 781039, India. senthilkumar@iitg.ac.in.

Funding

Department of Biotechnology, Ministry of Science and Technology, India BT/PR24592/NER/95/764/2017
6 · The paper itself

Abstract

Lactobacillus delbrueckii subsp. bulgaricus is a homofermentative lactic acid bacterium with recognized utility in dairy fermentation and unique capability to produce optically pure D-lactic acid (DLA), a critical monomer for polylactic acid-based bioplastics. Despite this potential, its industrial deployment has been constrained by poor galactose metabolism, incomplete lactose assimilation, and lack of efficient genetic tools. Here, we report the first comprehensive metabolic engineering of L. bulgaricus VI104 to establish a high-performance DLA cell factory. A tailored molecular toolbox was developed, including high-efficiency electroporation (~ 10

Indexed as

Lactic AcidLactobacillus delbrueckiiMetabolic EngineeringBacterial ProteinsFermentationGalactoseGene Expression Regulation, BacterialLactosePromoter Regions, GeneticBacterial ProteinsGalactoseLactic AcidLactoseD-lactate dehydrogenaseD-lactic acidGlycolytic genesLactic acid bacteriaLeloir pathwayMetabolic engineering

Identifiers

PMID40719898

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.