Triplet pregnancies after single blastocyst embryo transfer-how often and why do these occur?

Ariel Woods, Steven R Lindheim, Saira Kothari, Dean E Morbeck, Emily N Liu, Amy Zhu, Nigel Pereira

Abstract readCase Reports

In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ariel WoodsBaylor Scott and White Hospital, Department of Obstetrics and Gynecology, Temple, TX, USA.

Steven R LindheimUniversity of Central Florida College of Medicine, Orlando, FL, USA.

Saira KothariUniversity of Central Florida College of Medicine, Orlando, FL, USA.

Dean E MorbeckGenea Fertility, Sydney, Australia.

Emily N LiuQueen's University, Kingston, ON, Canada.

Amy ZhuMount Sinai Fertility, 250 Dundas Street W, #700, Toronto, ON, M5T 2Z5, Canada.

Nigel PereiraMount Sinai Fertility, 250 Dundas Street W, #700, Toronto, ON, M5T 2Z5, Canada. nigel.pereira@sinaihealth.ca.ORCID http://orcid.org/0000-0001-8160-5869

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single embryo transfer (sET) has been adopted by assisted reproductive technology (ART) clinics globally to increase the rate of singleton births and reduce multi-fetal pregnancies. Independent ART registry-based studies have reported an association between monozygotic (MZ) splitting and sET, especially after single blastocyst embryo transfer (sBET). While MZ twins after sBET are not uncommon, MZ triplet pregnancies after sBET are exceptionally rare. In this context, we report two cases of MZ triplet pregnancies after sBET. The first case is of a 29-year-old woman with a unicornuate uterus who conceived a tri-chorionic triplet pregnancy after single euploid blastocyst transfer in a medicated frozen embryo transfer (FET) cycle. The second case is of a 34-year-old woman with a triplet pregnancy consisting of monochorionic diamniotic twins and a singleton after the transfer of an untested blastocyst in a natural FET cycle. Using these cases, we focus on the epidemiology and putative mechanisms of MZ splitting. Given that sBET does not eliminate the risk of multiple pregnancy entirely, it is important to counsel couples about the risks of MZ twins and triplets with sBET.

Indexed as

BlastocystEmbryo TransferPregnancy, TripletSingle Embryo TransferAdultFemaleFertilization in VitroHumansPregnancyPregnancy, MultipleReproductive Techniques, AssistedTripletsTwins, MonozygoticMonozygotic splittingMultiple pregnancySingle blastocyst transferSingle embryo transfer

Identifiers

PMID40719807

DOI10.1007/s10815-025-03593-4

PMCPMC12423369

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.