ArticleNucleus (Austin, Tex.)2025
Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction.
Article in Nucleus (Austin, Tex.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The responsive nucleus: morphological signatures of cellular state.Nucleus (Austin, Tex.) · 2026Review
- Mechanical regulation of cell memory.Nature structural & molecular biology · 2026Review
- Review
- Quantitative Analysis of Plasmid DNA Distribution and Transgene Expression Following Cell Squeezing.Annals of biomedical engineering · 2026Article
- Changes in nuclear and actin mechanics from G1 to G2 affect nuclear integrity.Journal of cell science · 2026Article
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- H3K9me3 controls epidermis morphogenesis by regulating RNA Pol II dynamics at developmental promoters and enhancers.Nature communications · 2026Article
- Nuclear Mechanotransduction Across the Metastatic Cascade: Decoding Spatiotemporal Heterogeneity in Cancer Dissemination.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- AATF supports proliferation of glioblastoma cells by sustaining mitochondrial respiration through an NRF-1-dependent mechanism.Cell death & disease · 2026Article
- Nuclear Mechanics and Nuclear Mechanotransduction in Cancer Cell Migration and Invasion.Biomolecules · 2026Review
- Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation.bioRxiv : the preprint server for biology · 2026Article
- Topoisomerase I inhibition suppresses nuclear blebbing via RNA Pol II stalling and nuclear stiffening.bioRxiv : the preprint server for biology · 2026Article
- Setting Up to Divide: Nuclear Mechanotransduction in the Regulation of Mitosis.Results and problems in cell differentiation · 2026Review
- Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels.npj biological physics and mechanics · 2026Article
- Heterogeneity as a feature: unraveling chromatin's role in nuclear mechanics.Nucleus (Austin, Tex.) · 2025Review
- How the chromatin landscape influences nuclear morphology.Frontiers in cell and developmental biology · 2025Review
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Authors and funding
5 authors.
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Abstract
Aberrant nuclear morphology is a hallmark of human disease and causes nuclear dysfunction. Perturbed nuclear mechanics via reduced heterochromatin weakens the nucleus resulting in nuclear blebbing and rupture. While the role of heterochromatin is known, the separate roles of constitutive heterochromatin methylation states remains elusive. Using MEF and HT1080 cells, we isolated the individual contribution of constitutive heterochromatin H3K9 methylation states through histone methyltransferase inhibitors. Inhibition of SUV39H1 via Chaetocin downregulates H3K9 trimethylation (me3), while inhibition of G9a via BIX01294 downregulates H3K9 dimethylation (me2). Overall, the loss of H3K9me3 increased nuclear blebbing and rupture in interphase nuclei due to decreased nuclear rigidity from decompaction of chromocenters. Oppositely, loss of H3K9me2 decreased nuclear blebbing and rupture with increased nuclear rigidity and more compact chromocenters. We show that facultative heterochromatin and HP1α are non-essential for chromocenter compaction. Constitutive heterochromatin provides essential nuclear mechanical support to maintain nuclear shape and integrity through chromocenter compaction.
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