Evidence map›Paper›PMID 40718384›Full record

ArticleBiochemistry and biophysics reports2025

Alkaline phosphatase-streptavidin conjugate (APSA) enzyme and binding activity over time and storage conditions.

Nan Cheng, Lloyd Johnson, Jaimie Dufresne, Sina Mazinani, John G Marshall

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nan ChengResearch Analytical Biochemistry Laboratory, Department of Chemistry and Biology, Faculty of Science, Toronto Metropolitan University, 350 Victoria Street, Toronto, Ontario, M5B 2K3, Canada.
Lloyd JohnsonResearch Analytical Biochemistry Laboratory, Department of Chemistry and Biology, Faculty of Science, Toronto Metropolitan University, 350 Victoria Street, Toronto, Ontario, M5B 2K3, Canada.
Jaimie DufresneResearch Analytical Biochemistry Laboratory, Department of Chemistry and Biology, Faculty of Science, Toronto Metropolitan University, 350 Victoria Street, Toronto, Ontario, M5B 2K3, Canada.
Sina MazinaniResearch Analytical Biochemistry Laboratory, Department of Chemistry and Biology, Faculty of Science, Toronto Metropolitan University, 350 Victoria Street, Toronto, Ontario, M5B 2K3, Canada.
John G MarshallResearch Analytical Biochemistry Laboratory, Department of Chemistry and Biology, Faculty of Science, Toronto Metropolitan University, 350 Victoria Street, Toronto, Ontario, M5B 2K3, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alkaline phosphatase (AP) linked to streptavidin (SA) in the form of the APSA enzyme conjugate is required for diagnostic screening for a variety of clinical conditions world wide. The enzyme activity of APSA conjugates in the liquid phase showed variation across samples that declined with storage time. Random sampling of the enzyme activity in the liquid phase (ANOVA p < 2E-16; Regression p < 0.043) and the binding plus enzyme activity of APSA in the model assay (R

Indexed as

APSA, alkaline phosphatase streptavidin conjugateBCIP/NBTFreezing, freeze dryingGlycerol, sucrose, trehaloseProtease inhibitorsStorage conditions

Identifiers

PMID40718384
PMCPMC12296546

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.