Evidence map›Paper›PMID 40718055›Full record

ArticleTransplantation direct2025

Establishing and Validating a Biomolecular Signature of Ischemia/Reperfusion Injury in a Porcine Pancreas Allotransplantation Model.

Samrat Ray, Sujani Ganesh, Laura Martinez-Arenas, Catherine Parmentier, Masataka Kawamura, Bhranavi Arulratnam, Christian Hobeika, Emmanuel Nogueira, Francisco Calderon Novoa, Markus Selzner and 1 more

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Article in Transplantation direct, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Samrat RayAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Sujani GaneshAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Laura Martinez-ArenasHepatology, Hepatobiliopancreatic Surgery and Transplant Laboratory, Instituto de Investigacion Sanitaria La Fe, Universitat Politecnica de Valencia, CIBEREHD, Valencia, Spain.
Catherine ParmentierAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Masataka KawamuraAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Bhranavi ArulratnamAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Christian HobeikaAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Emmanuel NogueiraAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Francisco Calderon NovoaAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Markus SelznerAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.
Trevor W ReichmanAjmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Despite considerable advancement in surgical and immunological management in pancreas transplantation, graft pancreatitis remains a feared complication after pancreas transplantation. Identification of molecular mechanisms of underlying ischemia/reperfusion injury (IRI) in pancreas transplantation could, therefore, pave the path for targeted therapy to improve surgical outcomes. The aim of the study was to identify and validate the genes differentially expressed in the early period (24 h) of graft reperfusion in pancreas transplantation. Methods: A porcine pancreas allotransplant model (n = 4) was used to identify and validate the genes aberrantly expressed in 60 min postreperfusion tissue samples (phase 1). Trends of expression of selected genes from phase 1 and corresponding protein product levels in serum were validated at defined time points for >24 h in a technically replicated external cohort (n = 3; phase 2). Results: A total of 104 genes were found to be upregulated at 60 min after pancreas graft reperfusion. The most consistently overexpressed genes were IL6, THBS1, and MIR-21 (micro-RNA) mapped to protein kinase and intracellular signaling molecular pathways. Levels of expression of these genes correlated significantly with serum interleukin-6 ( Conclusions: The results provide new insights into biomolecular pathways (THBS1-IL6-MIR-21 crosstalk and hydroxymethylglutarate coenzyme A reductase-linked nuclear factor kappa B activation) linked to pancreatic IRI in porcine transplantation model. Identification and validation of some novel molecular pathway interactions in human pancreas transplantation could pave the path for potential targeted therapy in alleviating graft injury in the early phases of pancreatic IRI.

Identifiers

PMID40718055
PMCPMC12289139

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