ArticleAnalytical and bioanalytical chemistry2025
Enhanced immunochromatographic assay of tetracycline using intrinsic peroxidase-like activity of gold nanoparticles.
Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Tetracycline (TC) is a broad-spectrum antibiotic widely used in animal husbandry that may contaminate foodstuffs. In this study, a highly sensitive immunochromatographic assay (ICA) for TC was developed and applied to test meat samples. The assay utilized an indirect competitive format, with conjugates of anti-species antibodies and gold nanoparticles (GNPs) used to label specific immune complexes. The intrinsic peroxidase-like activity of GNPs was leveraged to enhance assay sensitivity. GNPs catalyzed substrate oxidation, resulting in a colored product that increased the brightness of the analytical zones on the test strips. An optimal substrate solution based on 3,3',5,5'-tetramethylbenzidine was selected. The limit of TC detection reached was 0.03 ng/mL. The assay duration was 18 min, including the catalytic reaction. Two meat sample preparation procedures were compared. The developed ICA demonstrated TC recoveries in beef ranging from 92 to 113%. The simple, rapid, and sensitive TC detection, combined with the use of GNP labels well-suited to manufacturing technologies, makes the developed ICA an efficient tool for mass screening tests of food products.
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