Evidence map›Paper›PMID 40715784›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2025

High pathogenic potential of Shigella flexneri strains descending from a common ancestor isolated over 34 years in Brazil.

Júlia Cunha Gonzales, Carolina Nogueira Gomes, Fábio Campioni, Dália Dos Prazeres Rodrigues, Ludmilla Tonani, Mário Henrique Paziani, Márcia Regina von Zeska Kress, Paulo da Silva, Juliana Pfrimer Falcão

Abstract read
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In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Júlia Cunha Gonzales *Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo- USP, Avenida Avenida do Café, s/n- Bloco S, sala 41, Ribeirão Preto- São Paulo, CEP 14040- 903, Brazil.
Carolina Nogueira Gomes *Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo- USP, Avenida Avenida do Café, s/n- Bloco S, sala 41, Ribeirão Preto- São Paulo, CEP 14040- 903, Brazil.
Fábio Campioni *Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo- USP, Avenida Avenida do Café, s/n- Bloco S, sala 41, Ribeirão Preto- São Paulo, CEP 14040- 903, Brazil.
Dália Dos Prazeres RodriguesFundação Oswaldo Cruz, Av. Brasil, Rio de Janeiro, 4365, CEP: 21040-900, Manguinhos, Brazil.
Ludmilla TonaniFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo- USP, Avenida Avenida do Café, s/n- Bloco S, sala 41, Ribeirão Preto- São Paulo, CEP 14040- 903, Brazil.
Mário Henrique PazianiFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo- USP, Avenida Avenida do Café, s/n- Bloco S, sala 41, Ribeirão Preto- São Paulo, CEP 14040- 903, Brazil.
Márcia Regina von Zeska KressFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo- USP, Avenida Avenida do Café, s/n- Bloco S, sala 41, Ribeirão Preto- São Paulo, CEP 14040- 903, Brazil.
Paulo da SilvaInstituto Adolfo Lutz de Ribeirão Preto, Rua Minas, 877, Ribeirão Preto-São Paulo, CEP: 14085-410, Brazil.
Juliana Pfrimer FalcãoFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo- USP, Avenida Avenida do Café, s/n- Bloco S, sala 41, Ribeirão Preto- São Paulo, CEP 14040- 903, Brazil. jufalcao@fcfrp.usp.br.ORCID http://orcid.org/0000-0002-1459-9355

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 140227/2019Conselho Nacional de Desenvolvimento Científico e Tecnológico 304399/2018-3Conselho Nacional de Desenvolvimento Científico e Tecnológico 304803/2021-9Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/716-3Fundação de Amparo à Pesquisa do Estado de São Paulo 2019/19338-8
6 · The paper itself

Abstract

Shigellosis is a serious public health problem worldwide caused by Shigella spp. The aims of this study were to molecularly and phenotypically characterize 50 S. flexneri strains isolated from human feces between 1983 and 2017 in different States of Brazil. The pathogenic potential of the strains studied was verified by the ability of the strains to invade human gut epithelial cells (Caco-2), as well as to survive in human macrophages (U-937). The virulence of eight S. flexneri strains, representative of the decades analyzed, was evaluated using the Galleria mellonella infection model. The phylogeny of the 50 strains was verified by Multilocus Sequence Typing (MLST). The percentage of invasion in Caco-2 cells was ≥ 40% and survival in U-937 was ≥ 60% for all 50 S. flexneri strains. Seven of the eight S. flexneri strains caused the death of more than 50% of the Galleria mellonella larvae. All 50 strains were typed as ST245 by MLST. In conclusion, the percentage of cell invasion, macrophage survival and G. mellonella mortality by the strains studied highlighted their pathogenic potential and virulence. The MLST result suggested the presence of phylogenetically related S. flexneri strains circulating in Brazil.

Indexed as

Dysentery, BacillaryShigella flexneriAnimalsBrazilCaco-2 CellsEpithelial CellsFecesHumansLarvaMacrophagesMothsMultilocus Sequence TypingPhylogenyVirulenceBacterial pathogenicityCell invasionGalleria mellonellaMLSTShigella flexneriShigellosis

Identifiers

PMID40715784
PMCPMC12350888

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.