Evidence map›Paper›PMID 40715532›Full record

ArticleAnalytical and bioanalytical chemistry2026

Comparative evaluation of reversed-phase and hydrophilic interaction liquid chromatography columns for untargeted profiling of bioactive compounds in Hypericum perforatum.

Davide Barboni, Desiree Bozza, Damiana Natasha Spadafora, Nicoletta Bianchi, Brunilda Myftari, Paola Tedeschi, Chiara De Luca, Simona Felletti, Matteo Spedicato, Alberto Cavazzini and 1 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Davide BarboniDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Desiree BozzaDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Damiana Natasha SpadaforaDepartment of Environmental and Prevention Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Nicoletta BianchiDepartment of Translational Medicine, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Brunilda MyftariDepartment of Pharmacy, University of Medicine, Rruga e Dibrës 371, Tirana, Albania.
Paola TedeschiDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Chiara De LucaDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Simona FellettiDepartment of Environmental and Prevention Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Matteo SpedicatoDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Alberto CavazziniDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Martina CataniDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy. martina.catani@unife.it.

Funding

Ministero dell'Università e della Ricerca 0001052Ministero dell'Università e della Ricerca P2022AJA7H8Ministero dell'Università e della Ricerca P2022PTYWP
6 · The paper itself

Abstract

The achievement of a comprehensive profiling of plant metabolites has long represented a challenge, not only due to their wide-ranging abundances but also as a result of their considerable chemical diversity. Recent advances in highly sensitive liquid chromatographic (LC) techniques, particularly when coupled with high-resolution mass spectrometry (HRMS), have established metabolomics as a key approach for the analysis of thousands of non-volatile metabolites in crude natural extracts. Nevertheless, the different polarities of primary and secondary metabolites often limit the efficacy of conventional reversed-phase liquid chromatography (RPLC) in providing exhaustive compound coverage. To address this limitation, orthogonal separation techniques such as hydrophilic interaction liquid chromatography (HILIC) should be employed as a complement to RPLC. In this work, four columns with identical geometrical specifications but with different stationary phase chemistry (one reversed-phase C18 and three different HILIC adsorbents) were employed for the untargeted analysis of bioactive compounds contained in Hypericum perforatum. The columns were evaluated not only in terms of chromatographic performance but also based on their ability to resolve challenging isobaric compound pairs of isobaric compounds. Finally, the integration of RPLC and HILIC data enabled a more comprehensive characterization of the metabolites associated with the plant.

Indexed as

Chromatography, Reverse-PhaseHypericumPlant ExtractsChromatography, High Pressure LiquidChromatography, LiquidHydrophobic and Hydrophilic InteractionsMass SpectrometryMetabolomicsPlant ExtractsHigh-resolution mass spectrometryHydrophilic interaction liquid chromatographyHypericum perforatumReversed-phase liquid chromatographyUntargeted analysis

Identifiers

PMID40715532
PMCPMC12783305

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.