ArticleMicrobial cell factories2025
Enhanced production of extracellular L-asparaginase in batch culture via nitrous acid-induced mutagenesis of Aspergillus oryzae.
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Pleurotus ostreatus L-asparaginase's use in food safety and biotechnology: from processing assistance to bioactive agent.Microbial cell factories · 2026Article
- Marine-derived L-asparaginase: unlocking marine power in anti-tumor therapeutics.Frontiers in immunology · 2025Review
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Authors and funding
11 authors.
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Abstract
backgroundL-Asparaginase (LA) is an important enzyme with therapeutic and industrial applications, particularly in the treatment of leukemia. Enhancing its production through optimization and strain improvement is crucial for commercial viability. This study aimed to increase LA production using Aspergillus oryzae by optimizing process parameters and employing chemical mutagenesis for strain improvement.
resultsAmong thirty-five fungal strains isolated from soil, ISL-3 A. oryzae and ISL-9 A. niger were identified as the most efficient LA producers. Using sugarcane bagasse as a substrate for solid-state fermentation, process optimization revealed that ISL-3 showed 12.15% higher yield than ISL-9 under the conditions of 5 g substrate level, 9 mL diluent MC-5, and 72 h of incubation. Chemical mutagenesis using nitrous acid resulted in the mutant NA-t3 with LA activity of 4.479 ± 0.22 U/g, significantly surpassing the parent strain. Inducible resistance was achieved on NA-cysL-C4 with 8 ppm L-cysteine HCl. Supplementation with MgSO
conclusionThe study successfully enhanced LA production from Aspergillus oryzae through process optimization and strain improvement using chemical mutagenesis. The significantly higher yield from the mutant strain makes it a promising candidate for commercial enzyme production. ANN was also employed on results to develop a correlation between experimental and predicted results. These findings highlight the potential of optimized solid-state fermentation and genetic enhancement techniques in industrial-scale.
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