ArticleBMC plant biology2025
Development and application of a cost-effective multiplex Kompetitive Allele-Specific polymerase chain reaction assay for pyramiding resistant genes of fusarium head blight and powdery mildew in wheat.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- High resolution mapping and development of diagnostic KASP markers for FHB resistance QTL on chromosome 1B in an elite soft red winter wheat cultivar 'Jamestown'.Molecular breeding : new strategies in plant improvement · 2026Article
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6 authors.
Funding
Abstract
backgroundWheat (Triticum aestivum L.) cultivation suffers from significant yield loss owing to diseases such as Fusarium head blight (FHB) and powdery mildew (PM). Utilization of host resistance is an effective strategy for controlling diseases. Fhb7 and Pm21 are derived from wild relatives of wheat, which confer broad-spectrum resistance to FHB and PM, respectively, and can be used in breeding through marker-assisted selection. Kompetitive Allele Specific polymerase chain reaction (KASP) is a homogeneous fluorescence-based technology, which identifies single nucleotide polymorphisms (SNP), and is suitable for marker-assisted selection (MAS) in large-scale breeding. However, KASP typically identifies the alleles of a single SNP, which limits the efficiency of pyramiding multiple genes during breeding.
resultsIn this study, we developed and applied a novel multiplex KASP (Multi-KASP) system that enabled concurrent detection of two distinct genes in a single reaction. The Multi-KASP system increased the flexibility of primer requirements and differentiated genes using only basic fluorescent cassettes, thereby significantly enhancing genotyping efficiency while ensuring high specificity and accuracy. This system accurately distinguished homozygous and heterozygous genotypes for both genes, which was validated by comparison with conventional marker detection. Phenotyping showed that polymerization of Fhb7 and Pm21 using the multi-KASP system enhanced resistance to FHB and PM in the wheat breeding program.
conclusionsA cost-effective Multi-KASP genotyping system, capable of simultaneously detecting two distinct genes in a single reaction, is an efficient and convenient solution for molecular breeding. The system demonstrates high potential for enhancing the efficiency of MAS in wheat breeding for resistance to FHB and PM.
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