ArticleMagnetic resonance in medicine2025
Impact of tissue sample preparation methods on myelin-sensitive quantitative MR imaging.
Article in Magnetic resonance in medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- Quantitative Comparison of Multi-Echo Spin Echo and Multi-Echo Gradient Echo Myelin Water Imaging in a Panel of Mbp Enhancer-Edited Mouse Lines.Magnetic resonance in medicine · 2026Article
- The Impact of Gadolinium on Quantitative Myelin Metrics in Ex Vivo Spinal Cord.NMR in biomedicine · 2026Article
- Phantom- and simulation-based validation of combined diffusion relaxometry in ex vivo ADRD white matter.bioRxiv : the preprint server for biology · 2026Article
- Impact of tissue sample preparation methods on myelin-sensitive quantitative MR imaging.Magnetic resonance in medicine · 2025Article
- Impact of Tissue Sample Preparation Methods on Myelin-Sensitive Quantitative MR Imaging.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
- Update of
Authors and funding
7 authors.
Funding
Abstract
purposeValidation of quantitative MRI (qMRI) parameters with histology is often done with ex vivo fixed tissue samples. Freezing is another common form of tissue preservation, but the effects of freezing and thawing tissue on myelin-sensitive quantitative MRI parameters and their correlation with histology require further analysis.
methodsMyelin water imaging, off-resonance RF saturation magnetization transfer (MT), and selective inversion recovery MT MRI experiments were conducted on 14 fresh, thawed, and fixed sheep brain tissue samples to calculate various surrogate measures of myelin content. These measures were compared with luxol fast blue (LFB) histological stain results.
resultsFresh, thawed, and fixed tissue qMRI values correlated well with LFB. Thawed and fixed tissue exhibited modest increases, between 3% and 32%, for most qMRI parameter values compared to fresh. Histology results showed that thawed samples did not lose tissue integrity from the freezing process.
conclusionFreezing is a reasonable alternative tissue preservation method to fixation for use in qMRI analysis, but may differentially affect qMRI parameter values in regions with varying myelin content.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.