ArticleCell reports2025
Cryo-EM and cryo-ET reveal the molecular architecture and host interactions of mycobacteriophage Douge.
Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Cryo-EM structure of bacteriophage Nocturne116: insights into the architecture of c2-likeJournal of virology · 2026Article
- Cryo-EM reveals a conserved baseplate architecture and distinct accessory protein assemblies in mycobacteriophages Claus, Corndog and Mysterious.Research square · 2026Article
- Dynamic adhesion device of phage OE33PA drives Gram-positive host recognition.bioRxiv : the preprint server for biology · 2026Article
- All the world's a phage.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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Authors and funding
21 authors.
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Abstract
Recent reports highlight the efficacy of engineered mycobacteriophages to treat non-tuberculosis mycobacterial disease. Molecular insights into mycobacteriophage architecture and host interactions could allow structure-guided phage engineering to increase efficacy and broaden host range, but such information is currently unavailable. We describe the cryoelectron microscopy (cryo-EM) structure of mycobacteriophage Douge, which contains 1,105 protein subunits assembled into a complete siphophage and is coated with glycan-binding domains for mycobacterial cell surface interactions. When filled with viral genome, the channel spanning the connector, tail, and baseplate is sealed by tape measure proteins, providing a genome gating system and requiring limited structural changes for genome ejection upon phage-host contact. Nanometer-resolution cryoelectron tomography (cryo-ET) snapshots of phage-host interactions show that the baseplate remains attached to the mycobacterial outer membrane during viral genome ejection. This study reveals high-resolution structural details of this mycobacteriophage and its interaction with host glycans.
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