ArticleFood and environmental virology2025
Detection of Dengue virus RNA in Wastewater during a Local Epidemic in Central Italy (August-October 2024).
Article in Food and environmental virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Methodological Approaches to Dengue Virus Detection in Wastewater: A Systematic Review and Meta-Analysis of Positivity Rate.Viruses · 2026Pooled it
- Harnessing a Surface Water-Based Multifaceted Approach to Combat Zoonotic Viruses: A Rural Perspective from Bangladesh and China.Microorganisms · 2025Article
- Rotavirus Quantification and Genotyping in Wastewater: A Molecular Surveillance Study in Italy (2024-2025).Microorganisms · 2025Article
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Authors and funding
21 authors.
Funding
Abstract
Wastewater-based epidemiology (WBE) is a well-established tool for monitoring enteric and respiratory viruses. However, its potential application to vector-borne diseases, including dengue virus (DENV), remains largely underexplored. This study investigated the feasibility of using WBE to detect DENV serotype 2 RNA during the largest dengue outbreak recorded to date in Italy (216 confirmed and probable cases), that occurred in the Marche Region (central Italy) from August to October 2024. From 1 to 30 October, during the decline of the epidemic (30 reported cases), 27 wastewater samples were collected from wastewater treatment plants in the affected municipality of Fano and in the nearby urban center of Pesaro. Four viral RNA concentration methods were tested: polyethylene glycol/sodium chloride precipitation, Nanotrap
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