Imprint Desorption Electrospray Ionization Mass Spectrometry Imaging (IDESI-MSI) Reveals Absorption of Triclopyr-Based Herbicide in Plants and Mouse Organs.

Hanzhi Liu, Yunshuo Tian, Ruolun Wei, Yifan Meng, Richard N Zare

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Article in Metabolites, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 citing paper in PubMed.

Comparison of Mass Spectrometry Imaging by Desorption Electrospray Ionization (DESI) and Desorption Electro-Flow Focusing Ionization (DEFFI).Metabolites · 2026
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Authors and funding

5 authors.

Hanzhi LiuCollege of Letters and Science, University of California, Santa Barbara, CA 93106, USA.

Yunshuo TianCollege of Letters and Science, University of California, Santa Barbara, CA 93106, USA.

Ruolun WeiDepartment of Neurosurgery, School of Medicine, Stanford University, Stanford, CA 94305, USA.

Yifan MengDepartment of Chemistry, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0001-6897-3595

Richard N ZareDepartment of Chemistry, Stanford University, Stanford, CA 94305, USA.

Funding

United States Air Force Office of Scientific Research AFOSR FA9550-21-1-0170

6 · The paper itself

Abstract

backgroundUnderstanding the absorption and distribution of herbicides in plants and animal tissues is essential for assessing their potential risks to human health.

methodIn this study, we employed imprint desorption electrospray ionization mass spectrometry imaging (IDESI-MSI) to visualize in both vegetable and animal tissues the absorption of Roundup which is a widely used herbicide.

resultsUsing IDESI-MSI with a pixel size of 150 µm, we detected the herbicide alongside several endogenous metabolites on oil-absorbing films applied to carrot sections. Time-course experiments revealed progressive herbicide penetration into carrot tissue, with penetration depth increasing linearly over time at a rate of approximately 0.25 mm/h. In contrast, green pepper samples showed minimal herbicide infiltration, likely owing to their hydrophobic cuticle barrier. Additionally, mice fed with herbicide-treated carrots exhibited detectable levels of herbicide in liver and kidney tissues.

conclusionsThese findings highlight the utility of IDESI-MSI as a powerful analytical platform for the rapid evaluation of chemical migration and absorption in food and biological systems, with important implications for food safety and toxicological research.

Indexed as

herbicide accumulationimprint desorption electrospray ionization mass spectrometry imagingoil-absorbing film

Identifiers

PMID40710537

DOI10.3390/metabo15070437

PMCPMC12300929

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