Evidence map›Paper›PMID 40707472›Full record

ArticleNPJ vaccines2025

Precocious Eimeria magna transgenically expressing RHDV P2 subdomain induces immune responses in rabbits.

Wenxuan Chen, Jingxia Suo, Jiahua Kong, Chunxia Lu, Xiaomin Ge, Fang Yu, Xinming Tang, Xun Suo, Xianyong Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ vaccines, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wenxuan ChenState Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jingxia SuoState Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jiahua KongState Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Chunxia LuState Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xiaomin GeState Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Fang YuDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xinming TangKey Laboratory of Animal Biosafety Risk Prevention and Control (North) of MARA, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Xun SuoState Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xianyong LiuState Key Laboratory of Veterinary Public Health and Safety; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China. liuxianyong@cau.edu.cn.

Funding

China Agriculture Research System of MOF and MARA CARS-43National Key Research and Development Program of China 2023YFD1802400National Natural Science Foundation of China 32072884
6 · The paper itself

Abstract

Rabbit coccidiosis and rabbit haemorrhagic disease (RHD) pose major threats to the rabbit industry, causing significant economic losses. Developing a multivalent vaccine to concurrently protect rabbits against Eimeria and RHDV infections would provide dual protection through a single immunization protocol. Here, we utilized a precocious line of E. magna (EmagPWT) as a vaccine vector to express P2 subdomain of RHDV capsid protein VP60. We constructed three transgenic parasites expressing (i) RHDV1-P2 subdomain, (ii) RHDV2-P2 subdomain, and (iii) 2 copies of P2 subdomains from both RHDV1 and RHDV2. We found that all transgenic parasites elicited detectable neutralizing antibodies and robust mucosal immune response following secondary immunization. In conclusion, our results indicate genetically manipulated precocious Eimeria parasite expressing heterologous antigens, such as P2 subdomain, holds promise as a vector for developing a multivalent vaccine against RHD and Eimeria infections in rabbits.

Identifiers

PMID40707472
PMCPMC12290045

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.