Evidence map›Paper›PMID 40706777›Full record

ArticlePharmacological research2025

Combined dietary omega-3 and omega-6 fatty acids protect against hyperglycemia-associated retinopathy in neonatal mice.

Myriam Boeck, Hitomi Yagi, Pia Lundgren, Aldina Pivodic, Anders K Nilsson, Yan Zeng, Chuck T Chen, Taku Kasai, Deokho Lee, Shen Nian and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmacological research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Myriam BoeckDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Hitomi YagiDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, Japan.
Pia LundgrenThe Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg 416 85, Sweden.
Aldina PivodicThe Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg 416 85, Sweden.
Anders K NilssonThe Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg 416 85, Sweden.
Yan ZengDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Chuck T ChenDepartment of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada.
Taku KasaiCenter for Interdisciplinary Cardiovascular Sciences, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Deokho LeeDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Shen NianDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Victoria HirstDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Katherine NeilsenDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Chaomei WangDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Jeff LeeDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Mathew YuDepartment of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada.
Andrew McCutcheonDepartment of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada.
Sasha A SinghCenter for Interdisciplinary Cardiovascular Sciences, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Masanori AikawaCenter for Interdisciplinary Cardiovascular Sciences, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Center for Excellence in Vascular Biology, Division of Cardiovascular Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Richard P BazinetDepartment of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada.
Zhongjie FuDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Ann HellströmThe Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg 416 85, Sweden.
Lois Eh SmithDepartment of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: lois.smith@childrens.harvard.edu.

Funding

Dietary control of angiogenesis in retinopathy modelsR01EY017017 · NEI · BOSTON CHILDREN'S HOSPITAL · PI Zhongjie Fu, Lois Smith · 2006 to 2026
$11.8M
Mouse Neurodevelopmental Behavior CoreU54HD090255 · NICHD · BOSTON CHILDREN'S HOSPITAL · PI WOOLF, CLIFFORD J · 2016 to 2020
$5.2M
Pro-inflammatory activation of human macrophages regulated by lncRNAsR01HL149302 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI AIKAWA, MASANORI · 2019 to 2022
$2.9M
PARP9 and PARP14 in atherosclerosisR01HL126901 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI AIKAWA, MASANORI · 2016 to 2019
$2.4M
Serine control of retinal neovascularization in retinopathyR01EY032492 · NEI · BOSTON CHILDREN'S HOSPITAL · PI FU, ZHONGJIE · 2021 to 2025
$2.2M
Glucose/lipid metabolism and vessel development in phase I ROPR01EY030904 · NEI · BOSTON CHILDREN'S HOSPITAL · PI SMITH, LOIS · 2020 to 2023
$1.7M
NEI NIH HHS R01 EY017017NEI NIH HHS R01 EY030904NEI NIH HHS R01 EY032492NHLBI NIH HHS R01 HL126901NHLBI NIH HHS R01 HL149302NICHD NIH HHS U54 HD090255
6 · The paper itself

Abstract

Retinopathy of prematurity (ROP) with early vessel loss (Phase I) followed by uncontrolled vessel growth (Phase II) causes visual impairment in premature infants. Although supplementation with omega-3 (n-3) docosahexaenoic acid (DHA) alone shows mixed results in preventing ROP, supplementation with both n-3 DHA and n-6 arachidonic acid (ARA) in early postnatal life reduces severe ROP by 50 % (Mega Donna Mega study). In the Mega Donna Mega study, 146 (72.6 %) of 201 included infants had at least one hyperglycemic episode during the first 14 days of life, which is a strong ROP risk factor. We therefore evaluated the protective effects and mechanisms of combined dietary n-3 DHA and n-6 ARA in a neonatal mouse model of hyperglycemia-induced suppression of retinal vascular development (Phase I ROP). At postnatal day (P) 10, retinal vessel growth was improved in pups from mothers on diets enriched with 1 % DHA + 2 % ARA vs. 3 % DHA. Lipid changes in pup plasma and RPE complex (retinal pigment epithelium with choroid and sclera) were in accordance with maternal diets' DHA and ARA levels, indicating that milk lipids reflected maternal diets. Proteomic retinal analysis revealed increased abundances of proteins related to mitochondrial respiration and glucose metabolism with the combined diet. Inhibition of mitochondrial ATP synthase negated the protective effects of the combined diet. In conclusion, combined DHA+ARA oral maternal supplementation protects against hyperglycemia-induced retinopathy in mouse neonates (Phase I ROP model) through enhanced retinal metabolism, suggesting the potential of balanced lipid supplementation for ROP prevention.

Indexed as

Arachidonic AcidDocosahexaenoic AcidsFatty Acids, Omega-3Fatty Acids, Omega-6HyperglycemiaRetinopathy of PrematurityAnimalsAnimals, NewbornDietary SupplementsDisease Models, AnimalFemaleMaleMiceMice, Inbred C57BLRetinaRetinal VesselsArachidonic AcidDocosahexaenoic AcidsFatty Acids, Omega-3Fatty Acids, Omega-6ARADHAPostnatal hyperglycemiaRetinal vasculatureRetinopathy of prematurity

Identifiers

PMID40706777
PMCPMC12445345

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.