ArticleJournal of AOAC International2025
Validation of Enzytec™ Liquid Combi Lactose/D-Galactose for Enzymatic Determination of Lactose and D-Galactose in Selected Foods: Official Method 2024.10 First Action.
Article in Journal of AOAC International, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLactose is a disaccharide composed of D-galactose and D-glucose. Lactose composes 2-8% of milk by weight. Lactose is digested in the small intestine by the enzyme lactase. Lactase deficiency can lead to lactose intolerance in adults.
objectiveEnzytec™ Liquid Combi Lactose/D-Galactose is an enzymatic test kit for the determination of lactose and D-galactose in milk, milk products, infant formula, chocolate, sausage meat, almond milk, salad dressing, bread, and cookies. Excluded are lactose-free dairy products that have been enzymatically treated with lactase.
methodsThe kit contains two sets of reagents in a ready-to-use format and is suitable for automation. Lactose is cleaved with ß-galactosidase. Resulting D-galactose is oxidized by galactose-dehydrogenase and NAD+. The amount of resulting NADH is equivalent to the converted amount of lactose and free D-galactose. For measurement of free D-galactose alone the second set of reagents is used.
resultsTrueness of the lactose measurement was checked using certified reference materials with recoveries for milk, milk products, infant formula, and chocolate between 94 and 103%. Spiking experiments at low levels in almond milk, salad dressing, feta cheese, cookies, and bread revealed recoveries between 92 and 121%. Intermediate precision varied between 3 and 10.6% for lactose and between 6.5 and 7.8% for D-galactose. For automation, three applications with different test volumes were validated. Linearity is given from 12.5 mg/L to 12.5 g/L for lactose and 4.0 mg/L up to 6.25 g/L for galactose.
conclusionsThe method is robust and accurate for manual and automated applications. The method was approved as AOAC Official Method of AnalysisSM. HIGHLIGHTS: The ready-to-use components of the test kit have a shelf life of at least 24 months.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.