ArticleCurrent protocols2025
Nonenzymatic Primer Extension Experiments in the Presence of Coacervates.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Nonenzymatic Primer Extension Experiments in the Presence of Coacervates.Current protocols · 2025Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This article outlines a method for investigating the formation and function of potentially prebiotic complex coacervates, as primitive compartments, generated through electrostatic interactions between anionic nucleic acids and cationic peptides. The Basic Protocols specifically detail the experimental procedures for conducting nonenzymatic RNA primer extension reactions using 2-aminoimidazole-activated ribonucleoside 5'-O-monophosphates in the presence of these coacervates (e.g., composed of specific "host" DNA/RNA sequences and short polyarginine peptide partners). Designed to be accessible to researchers with limited prior experience in coacervate systems, this step-by-step guide provides a practical framework for probing prebiotic polymerization processes, an area of importance in the study of the origins of life. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Screening for suitable host DNA/RNA sequences and cationic peptide sequences Basic Protocol 2: Primer extension in the presence of coacervates.
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Registered trials
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