Evidence map›Paper›PMID 40704575›Full record

ArticleJournal of cell science2025

Creation and validation of a proteome-wide yeast library for protein detection and analysis.

Din Baruch, Ioannis Tsirkas, Ehud Sass, Benjamin Dubreuil, Yeynit Asraf, Amir Aharoni, Maya Schuldiner, Ofir Klein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Din BaruchDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.ORCID 0000-0003-0932-1890
Ioannis TsirkasDepartment of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, 84105, Israel.
Ehud SassDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Benjamin DubreuilDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Yeynit AsrafDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Amir AharoniDepartment of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, 84105, Israel.
Maya SchuldinerDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.ORCID 0000-0001-9947-115X
Ofir KleinDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.ORCID 0000-0002-9635-4481

Funding

Chan Zuckerberg Initiative 2023-331952European Research Council OnTarget 864068H2020 European Research Council OnTarget 864068Weizmann Institute of Science
6 · The paper itself

Abstract

A significant challenge in cell biology is to uncover the function of uncharacterized proteins. Surprisingly, a quarter of the proteome is still poorly understood even in the most well-studied model organisms. Systematic methodologies, including the use of tagged protein collections, have emerged as a powerful approach to address this gap. Despite the availability of proteome-wide collections featuring various fused proteins, the impact of different tags on protein function highlights the need for diversifying the tags used for functional genomic studies. To rise to this challenge, we created a proteome-wide collection of yeast strains in which proteins are N-terminally tagged with the broadly utilized and compact hemagglutinin (HA) epitope. We showcase the potential uses of our library for systematically evaluating protein size, abundance and localization using an in vivo labeling approach. Our characterization underscores the potential utility of a proteome-wide HA-tagged library in revealing novel aspects of cell biology, providing an additional powerful tool for functional genomics.

Indexed as

Gene LibraryProteomeProteomicsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsProteomeSaccharomyces cerevisiae ProteinsHA tagProteome-wide librarySaccharomyces cerevisiaescFvSingle-chain fragment variableSWAT approach

Identifiers

PMID40704575
PMCPMC12450460

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.