ArticleScientific reports2025
Differential decay of multiple environmental nucleic acid components.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Methodological Approaches to Dengue Virus Detection in Wastewater: A Systematic Review and Meta-Analysis of Positivity Rate.Viruses · 2026Pooled it
- Biomonitoring 3.0: Beyond Taxa Lists to Evidence-Tiered Monitoring of Ecosystem Dynamics and Interactions.Ecology and evolution · 2026Review
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- Vertebrate biodiversity via eDNA at the air-water interface.iScience · 2026Article
- Passive air sampling detects environmental DNA transfer from water into air.Scientific reports · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Detections of environmental nucleic acids (eNA), such as DNA and RNA, are powerful tools for monitoring biodiversity. Yet, precise interpretation of these indirect detections requires understanding of eNAs persistence. We conducted a decay experiment to track degradation of six eNA components derived from the bottlenose dolphin Tursiops truncatus: mitochondrial eDNA of varying lengths, ribosomal eRNA, and messenger eRNA. Target eNAs were quantified over seven days via digital droplet PCR (ddPCR). Decay followed a biphasic exponential model with rapid initial loss (~ 24 h at 15 °C), followed by slower degradation. Mitochondrial messenger eRNA was least stable, disappearing within four hours. Ribosomal eRNA persisted longer but degraded slightly faster than its eDNA counterpart (decay rate λ₁ = 0.236 vs. 0.165 h⁻¹). Longest eDNA fragments decayed more rapidly (λ₁ = 0.190 h
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.