ArticleScientific reports2025
LPS promotes the production of ROS in neutrophils to regulate their killing activity against Mycobacterium tuberculosis.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- MXene quantum dots for immunomodulation: redox activity and nano-strategies in inflammatory control and regenerative medicine.RSC advances · 2026Review
- Dual-Targeted Nanotherapy Restores Redox Homeostasis and Suppresses Uterine Hypercontractility for Effective Preterm Birth Intervention.Advanced healthcare materials · 2026Article
- Context-specific roles for IL-17 in tuberculosis.PLoS pathogens · 2026Review
- Evaluating lymphocyte change rate and lactate as predictors of prognosis in critical COVID-19 patients in the intensive care unit.Frontiers in cellular and infection microbiology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Tuberculosis (TB) is now the leading cause of death globally from a single infectious disease. So far, the exact mechanism of anti-tuberculosis immunity has not been fully elucidated, and the immune role of neutrophils in anti-tuberculosis infection is controversial. We investigated the killing function of neutrophils against Mycobacterium tuberculosis (M.tb) and the effect of neutrophils activated by lipopolysaccharide (LPS) on the production of reactive oxygen species (ROS), to evaluate the mechanism by which neutrophils eradicate M.tb mediated infection and find theoretical basis for clinical treatment of tuberculosis. The killing rate of neutrophils to FDA (Fluorescein diacetate)-labeled M.tb was detected by flow cytometry, and the killing rate of neutrophils to M.tb was observed by fluorescence microscopy. The activation rate and ROS production of neutrophils were observed at different time points after M.tb infection. Flow cytometry was utilized to detect the effect of LPS on the ROS production during neutrophil mediated killing of M.tb. Toll-like receptor 4 (TLR4) monoclonal antibody or NADPH oxidase inhibitor was utilized to detect the LPS activated neutrophil mediated production of ROS during M.tb killing. The killing function of neutrophils against M.tb increased with time. The activation rate and ROS production of neutrophils increased with time after M.tb infection. The activation rate and ROS production of neutrophils increased with the increase of LPS concentration. The activation rate and ROS production of PMN were reduced by TLR4 monoclonal antibody or NADPH oxidase inhibitor.LPS-TLR4 pathway is involved in neutrophils induced ROS mediated killing of M.tb. LPS promotes neutrophils mediated killing of M.tb, through ROS mediated production of NADPH oxidase which provides a theoretical basis for testing the role of neutrophils in clearance of M.tb in humans, and reducing the M.tb pathogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.