ArticleScience advances2025
Photoacoustic and fluorescence hybrid microscope for cortex-wide imaging of neurovascular dynamics with subcellular resolution.
Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- MIMAS: An open-source microlens-array miniature microscope for accessible large-field cortical imaging in freely behaving mice.Science advances · 2026Article
- Photoacoustic and ultrasound Imaging of placental functions in gestational diabetes: From diagnosis to precision interventions.Photoacoustics · 2026Review
- Optoacoustic brain imaging: A systematic review and field mapping.Photoacoustics · 2026Article
- Simultaneous mesoscopic imaging of neural activity and hemodynamics reveals anesthetic modulation of neurovascular responses.Photoacoustics · 2026Article
- Robust hybrid feature-driven on-the-fly mapping enables freehand 3D panoramic photoacoustic angiography.Light, science & applications · 2026Article
- Application Mechanisms and Clinical Prospects of Brain-Computer Interface Technology in Radiation-Induced Brain Injury.Cellular and molecular neurobiology · 2026Review
- PEC-DFM Hybrid-Sensor Based on Nanoparticle-Resolved Estimation for Sensitive and Reversible Glucose Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Photon absorption remote sensing (PARS): comprehensive absorption imaging enabling label-free biomolecule characterization and mapping.Scientific reports · 2026Article
- Dysfunction of the neurovascular unit as a temporal driver in Alzheimer's pathogenesis.Translational neurodegeneration · 2026Review
- Inducing and monitoring photothrombotic stroke in anesthetic neuroprotection-free mice using functional photoacoustic microscopy.Neurophotonics · 2026Article
- Photoacoustic Microscopy for Multiscale Biological System Visualization and Clinical Translation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Spectral-distortion-suppressed deep learning for fiber sensor photoacoustic microscopy.Photoacoustics · 2025Article
- Hybrid Fourier-Derivative Analysis: An accurate and fast method for blood flow quantification in photoacoustic microscopy.Photoacoustics · 2025Article
- A duet: Seeing and hearing the brain in action.Science advances · 2025Review
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Authors and funding
23 authors.
Funding
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Abstract
Simultaneously monitoring cortex-wide microvascular dynamics, blood oxygenation metabolism, and neuronal activities with high spatiotemporal resolution is essential for understanding neurovascular coupling (NVC). However, it remains a challenge for existing neuroimaging tools. Here, we report a linear transducer array-based hybrid microscope (LiTA-HM) that integrates photoacoustic microscopy and confocal fluorescence microscopy to achieve simultaneous neurovascular imaging with a field of view (FOV) of 6 millimeters by 5 millimeters, a spatial resolution of ~6 micrometers, and a temporal resolution of 0.8 seconds. We design an array of eight miniature transducers to enlarge FOV and optimize a polygon mirror-based ultrafast scanner to achieve real-time imaging. Proof-of-concept imaging experiments were conducted on awake mice. Different couplings between thousands of neuron somas and vessel branches down to capillaries were observed in hypoxia and anesthesia experiments. Spatiotemporal correlations and functional connectivity in NVC were uncovered for spreading depolarization waves occurred in a drug-induced epilepsy experiment. We expect that LiTA-HM will enable broad applications of neuroimaging.
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